一株红球菌脱硫菌株脱硫特性的研究

化学学报 ›› 2004, Vol. 62 ›› Issue (19): 1883-1888. 上一篇下一篇

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一株红球菌脱硫菌株脱硫特性的研究

马翠卿¹, 佟明友², 于波¹, 曾义勇¹, 方向晨², 冯进辉¹, 张全², 李福利¹, 许平¹

1. 山东大学微生物技术国家重点实验室, 济南, 250100;
2. 中国石油化工股份有限公司抚顺石油化工研究院, 抚顺, 113001

投稿日期:2003-12-08 修回日期:2004-06-07 发布日期:2014-02-17
通讯作者: 许平,E-mail:pingxu@sdu.edu.cn E-mail:pingxu@sdu.edu.cn
基金资助:
国家自然科学基金项目(Nos.29977011,30270045)、中国石油化工股份有限公司重点项目(No.1010807)、国家十五科技攻关项目(No.2001BA707B01)资助.

Biodesulfurization of Organic Sulfur by a Newly Isolated Rhodococcus sp.Strain SDUZAWQ

MA Cui-Qing¹, TONG Ming-You², YU Bo¹, ZENG Yi-Yong¹, FANG Xiang-Chen², FENG Jin-Hui¹, ZHANG Quan², LI Fu-Li¹, XU Ping¹

1. State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100;
2. Fushun Research Institute of Petroleum and Petrochemicals, SINOPEC, Fushun 113001

Received:2003-12-08 Revised:2004-06-07 Published:2014-02-17

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摘要/Abstract

从炼油厂污水排放口取得的土样中筛选到一株能降解二苯并噻吩(DBT)的菌株,用GC/MS方法,证明其降解DBT走硫专一脱除途径,即"4S途径".该菌株被命名为SDUZAWQ,用微生物生理生化实验及16S rDNA序列分析初步鉴定为红球菌属(Rhodococcus sp.).实验结果表明,红球菌SDUZAWQ可有效降解苯并噻吩(BT)和DBT及其甲基衍生物.在2 d内,BT与DBT可同时完全降解,0.5 mmol·L^-1的BT在1 d内可降解掉86%,降解速度高于DBT的降解.5-MBT的降解率也高于4,6-DMDBT和4-MDBT.4-MDBT较4,6-DMDBT更难降解,在2 d内,红球菌SDUZAWQ可降解62%的4,6-DMDBT,而相同条件下,4-MDBT仅能被降解36%.

关键词: 二苯并噻吩(DBT), 苯并噻吩(BT), 脱有机硫, 代谢途径

A strain named SDUZAWQ was isolated from soil contaminated by waste water of a refinery. Using GC/MS method, the strain was determined to biodesulfurize dibenzothiophene (DBT) via a sulfur-specific pathway, as so-called "4S pathway". This culture was identified as Rhodococcus sp. based on a partial 16S rDNA gene sequence and physiological and biochemical methods. Rhodococcus sp. SDUZAWQ can effectively desulfurize benzo[b]thiophene (BT), DBT and their derivatives. In two days, BT and DBT were degraded completely and 86% of BT was desulfurized in 24 h with the degrading rate being higher than that of DBT. The rate of 5-methyl-benzo[b]thiophene (5-MBT) was also higher than those of DBT derivatives, 4,6-dimethyldibenzothiophene (4,6-DMDBT) and 4-methyl-dibenzothiophene (4-MDBT). The degradation of 4-MDBT was more refractory than that of 4,6-DMDBT. In 48 h, 62% of 4,6-DMDBT was degraded, but only 36% of 4-MDBT was desulfurized under the same conditions.

Key words: dibenzothiophene, benzo[b]thiophene, biodesulfurization, metabolic pathway

引用此文

马翠卿, 佟明友, 于波, 曾义勇, 方向晨, 冯进辉, 张全, 李福利, 许平. 一株红球菌脱硫菌株脱硫特性的研究[J]. 化学学报, 2004, 62(19): 1883-1888.

MA Cui-Qing, TONG Ming-You, YU Bo, ZENG Yi-Yong, FANG Xiang-Chen, FENG Jin-Hui, ZHANG Quan, LI Fu-Li, XU Ping. Biodesulfurization of Organic Sulfur by a Newly Isolated Rhodococcus sp.Strain SDUZAWQ[J]. Acta Chimica Sinica, 2004, 62(19): 1883-1888.

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一株红球菌脱硫菌株脱硫特性的研究

Biodesulfurization of Organic Sulfur by a Newly Isolated Rhodococcus sp.Strain SDUZAWQ

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