DNA与非离子糖基表面活性剂相互作用的研究

化学学报 ›› 2004, Vol. 62 ›› Issue (6): 561-566. 上一篇下一篇

研究论文

DNA与非离子糖基表面活性剂相互作用的研究

敬登伟¹, 张剑^1,2, 张高勇^1,2

1. 中国日用化学工业研究院表面活性剂国家工程研究中心, 太原, 030001;
2. 太原理工大学化学与化工学院, 太原, 030032

投稿日期:2003-07-18 修回日期:2003-11-20 发布日期:2014-02-18
通讯作者: 张高勇,E-mail:RIDCI@public.ty.sx.cn E-mail:RIDCI@public.ty.sx.cn
基金资助:
国家自然科学基金(No.20233010)资助项目.

Study on the Interaction between Nonionic Sugar-based Surfactant and DNA

JING Deng-Wei¹, ZHANG Jian^1,2, ZHANG Gao-Yong^1,2

1. Research Institute of Daily Chemical Industry, Taiyuan 030001;
2. School of Chemistry and Chemical Engineering, Taiyuan University of Technology, Taiyuan 030002

Received:2003-07-18 Revised:2003-11-20 Published:2014-02-18

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摘要/Abstract

用动态表面张力法、键合等温线、紫外光谱及荧光光谱等方法研究了不同链长烷基葡萄糖苷(APG)与DNA的相互作用.研究发现APG对DNA键合可分为两阶段,第一阶段:多苷依靠多羟基结构与DNA形成动力学稳定的复合物;第二阶段:随时间延长,单苷由于其较小的空间位阻而与DNA形成能量更低的热力学稳定复合物.由平衡渗析法得到的单苷与DNA相互作用键合等温线显示,APG与DNA键合为一非协同过程,证实了其非离子氢键吸附的本质,同时也支持了DNA对胶束及预胶束的缠绕模型.紫外光谱结果证明了在APG与DNA作用过程中疏水作用的重要性.以溴化乙锭为探针,荧光光谱法研究证明,随APG链长增加, DNA构象缩拢程度加大,但即使是C₁₂APG也仅能使DNA构象部分缩拢,推测DNA仅是部分链段对APG胶束进行包裹,其它链段仍处于伸展状态.与阳离子表面活性剂十六烷基三甲基溴化铵(CTAB)可使DNA构象强烈缩拢的事实相比,证明了静电作用在大分子与表面活性剂相互作用中的主导性.

关键词: DNA, 糖基表面活性剂, 键合等温线, 荧光光谱

The interaction of DNA with alkyl polyglucoside of different lengths was investigated by dynamic surface tension measurement, binding isotherm, UV spectra and fluorescence probe method. It was found that the binding of APG on DNA chain could be divided into two stages. At the first stage, multiglucoside interacted with DNA via its multi hydroxyl head group and formed with DNA a kinetic stable complex; at the second stage, monoglucoside due to its small head and hence less steric hindrance formed a thermal stable complex with DNA. Binding isotherms obtained by monoglucoside and DNA showed that binding of APG on DNA chain was a non-cooperative process, demonstrating the hydrogen bonding nature of the binding process. This also supports the model that APG micelle is surrounded by part of DNA chain. UV adsorption results showed the importance of hydrophobic effect in the interaction between DNA and APG. With ethidium bromide (EB) as probe, it was found by fluorescence spectra that DNA conformation was more compacted as the alkyl chain of APG increased. However, even C₁₂APG could only partially compact DNA. Compared with the fact that CTAB could compact DNA into discrete globules, it was assumed that electrostatic effect was dominant in the interaction between DNA and surfactant.

Key words: DNA, sugar-based surfactant, binding isotherm, fluorescence spectrum

引用此文

敬登伟, 张剑, 张高勇. DNA与非离子糖基表面活性剂相互作用的研究[J]. 化学学报, 2004, 62(6): 561-566.

JING Deng-Wei, ZHANG Jian, ZHANG Gao-Yong. Study on the Interaction between Nonionic Sugar-based Surfactant and DNA[J]. Acta Chimica Sinica, 2004, 62(6): 561-566.

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DNA与非离子糖基表面活性剂相互作用的研究

Study on the Interaction between Nonionic Sugar-based Surfactant and DNA

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