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化学学报
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化学学报 ›› 2005, Vol. 63 ›› Issue (22): 2042-2046. 上一篇    下一篇

研究论文

碳纳米管修饰金电极检测特定序列DNA

唐婷*,1,2， 彭图治，时巧翠1   

  1. (1浙江大学化学系 西溪校区 杭州 310028)
    (2杭州师范学院医药卫生管理学院 杭州 310036)
  • 投稿日期:2005-03-23 修回日期:2005-08-02 发布日期:2010-12-10
  • 通讯作者: 唐婷

Sequence Determination of DNA Pieces Using Carbon Nanotube Modified Gold Electrodes

TANG Ting*,1,2, PENG Tu-Zhi, SHI Qiao-Cui1   

  1. (1 Department of Chemistry, Xixi Campus, Zhejiang University, Hangzhou 310028)
    (2 Medicine and Health Manage College of Hangzhou Teachers College, Hangzhou 310036)
  • Received:2005-03-23 Revised:2005-08-02 Published:2010-12-10
  • Contact: TANG Ting

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1824

被引次数

96

利用化学偶联法将末端修饰氨基的寡聚核苷酸固定在表面修饰有羧基化碳纳米管(CNTs-COOH)的金电极表面, 制备新型核酸探针, 可以特异性结合目标单链寡聚核苷酸. 以阿霉素作为嵌合指示剂, 利用示差脉冲法测定杂交的结果. 经过实验条件的优化, 测定DNA浓度在1.0×10－6～1.0×10－9 mol/L呈良好的线性关系. 检测限为: 2.54×10－10 mol/L. 碳纳米管特有的纳米结构对检测结果的放大作用, 提高了该传感器的检测限和灵敏度.

关键词: 羧基化碳纳米管, DNA杂交, 阿霉素, 示差脉冲伏安法

A novel electrochemical biosensor was reported for the detection of nucleic acid hybridization and sequence-specific DNAs. A synthesized single stranded DNA (ssDNA-1) was covalently immobilized on gold electrodes, which had been modified by carboxylic group-functionalized carbon nanotubes activated using N-ethyl-N'-(3-dimethylaminopropyl)carbodiimide(EDC) and N-hydroxysuccinimidobiotin (NHS). Differential pulse voltammetry was used to detect DNA with an electroactive indicator of adriamycin which could bind to double stranded DNA. The linear range for the determination of target DNA is from 1.0×10－6 to 1.0×10－9 mol/L, and the detection limit is found to be 2.54×10－10 mol/L.

Key words: carboxylic carbon nanotube, DNA hybridization, adriamycin, differential pules voltammetry