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化学学报
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化学学报 ›› 2006, Vol. 64 ›› Issue (16): 1700-1704. 上一篇    下一篇

研究论文

耐药相关果糖二磷酸醛缩酶C的生物质谱分析与鉴定

吕磊1,2,3, 刘志强*,1, 李丽1, 刘宁1, 刘淑莹1   

  1. (1中国科学院长春应用化学研究所 长春质谱中心 长春 130022)
    (2延安大学化学系 延安 716000)
    (3中国科学院研究生院 北京 100039)
  • 投稿日期:2005-12-14 修回日期:2006-01-09 发布日期:2006-08-28
  • 通讯作者: 刘志强

Identification and Analysis of Resistance-related Fruc-tose-bisphosphate Aldolase C by Bio-mass Spectrometry

LÜ Lei 1,2,3; LIU Zhi-Qiang*,1; LI Li 1; LIU Ning1; LIU Shu-Ying1   

  1. (1 Changchun Institute of Applied Chemistry, Changchun Center of Mass Spectrometry, Changchun 130022)
    (2 Department of Chemistry, Yan'an Univerisity, Yan'an 716000)
    (3 Graduate School of the Chinese Academy of Sciences, Beijing 100039)
  • Received:2005-12-14 Revised:2006-01-09 Published:2006-08-28
  • Contact: LIU Zhi-Qiang

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被引次数

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醛缩酶C是生命代谢过程中重要糖酵解同工酶之一. 应用二维凝胶电泳分离卵巢癌细胞中高表达的果糖二磷酸醛缩酶C, 并通过基质辅助激光解吸电离飞行时间串联质谱对其进行分析与鉴定, 结果表明应用高分辨二维凝胶电泳分离, 结合生物质谱联用技术分析和鉴定未知蛋白具有简单快捷的特点.

关键词: 醛缩酶C, 基质辅助激光解吸电离串联飞行时间质谱, 二维凝胶电泳

Fructose-bisphosphate aldolase C is one of important glycolytic isozymes in the metabolic process. The fructose-bisphosphate aldolase C which highly expressed in the human ovarian adenocarcinoma cell has been separated by two-dimensional gel electrophoresis and identified by matrix-assisted laser desorption ionization tandem time-of-flight mass spectrometry (MALDI-TOF/TOF MS). The results indicate that MALDI-TOF/TOF MS in combination with the high resolution two dimensional gel electrophoresis is a simple and fast method for identification of unknown proteins.

Key words: aldolase C, matrix-assisted laser desorption ionization tandem time of flight mass spectrometry, two dimensional gel electrophoresis