SIOC English
化学学报
高级检索

化学学报 ›› 2006, Vol. 64 ›› Issue (17): 1799-1804. 上一篇    下一篇

研究论文

TiO2纳米颗粒对钌(II)三联吡啶配合物光损伤小牛胸腺DNA能力的影响

丁慧颖1,2; 宋林青1; 陈景荣1; 王雪松*,1; 张宝文*,1   

  1. (1中国科学院理化技术研究所 北京 100101)
    (2中国科学院研究生院 北京 100039)
  • 投稿日期:2006-02-27 修回日期:2006-04-13 发布日期:2006-09-14
  • 通讯作者: 张宝文

Effects of TiO2 Nanoparticles on the Photodamage of CT-DNA by Ru(II) Bis(terpyridine) Complexes

DING Hui-Ying1,2; SONG Lin-Qing1; CHEN Jing-Rong1; WANG Xue-Song*,1; ZHANG Bao-Wen*,1   

  1. (1 Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100101)
    (2 Graduate School of the Chinese Academy of Sciences, Beijing 100039)
  • Received:2006-02-27 Revised:2006-04-13 Published:2006-09-14
  • Contact: ZHANG Bao-Wen

PDF

1030

由于极短的激发态寿命, 钌(II)三联吡啶配合物对脱氧核糖核酸(DNA)的光损伤能力低下. 设计合成了三个钌(II)三联吡啶配合物[Ru(ttp)(tpy)]2＋ (1), [Ru(ttp-COOH)(tpy)]2＋ (2)和[Ru(ttp-COOH)(tpy-pyr)]2＋ (3), 其中tpy为2,2':6',2"-三联吡啶, ttp为4′-(4-甲苯基)-2,2':6',2"-三联吡啶, ttp-COOH为4′-(4-羧基苯基)-2,2':6',2"-三联吡啶, tpy-pyr为4'-(1-芘基)-2,2':6',2"-三联吡啶. 比较了TiO2纳米颗粒对它们光损伤小牛胸腺DNA的影响. 发现TiO2纳米颗粒在空气和氩气条件下均可显著提高配合物3光损伤DNA的能力. TiO2纳米颗粒和配合物3间的光诱导电子转移作用及其该作用生成的钌(III)物种可能是促进配合物3对DNA光损伤的主要原因.

关键词: 钌配合物, 三联吡啶配体, 二氧化钛纳米颗粒, 光诱导电子转移, 小牛胸腺DNA, 光损伤

Due to their short excited state lifetimes, Ru(II) bis(terpyridine) complexes can hardly used as DNA photocleavers. In this paper three Ru(II) bis(terpyridine) complexes [Ru(ttp)(tpy)]2＋ (1), [Ru(ttp- COOH)(tpy)]2＋ (2) and [Ru(ttp-COOH)(tpy-pyr)]2＋ (3) were designed and synthesized (tpy＝2,2':6',2"- terpyridine, ttp＝4'-(p-tolyl)-2,2':6',2"-terpyridine, ttp-COOH＝4'-(p-carboxyl-phenyl)-2,2':6',2"-terpyridine, tpy-pry＝4'-(1-pyrenyl)-2,2':6',2"-terpyridine). It was found that TiO2 nanoparticles improved the photodamage ability of 3 on calf thymus DNA in both aerated and deaerated conditions. The oxidized form of 3, [Ru(III)(ttp-COOH)(tpy-pyr)]3＋, generated by photoinduced electron transfer between TiO2 nanoparticle and 3, was invoked to account for the enhanced DNA photodamage.

Key words: Ru(II) complex, terpyridine ligand, TiO2 nanoparticle, photoinduced electron transfer, calf thymus DNA, photodamage