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化学学报
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化学学报 ›› 2006, Vol. 64 ›› Issue (21): 2161-2164. 上一篇    下一篇

研究论文

纳米二氧化钛对卵巢肿瘤细胞的选择性凋亡的诱导作用

朱融融, 汪世龙*,陈小平, 孙晓宇, 张蕤, 姚思德   

  1. (同济大学生命科学与技术学院 上海 200092)
  • 投稿日期:2006-04-26 修回日期:2006-06-06 发布日期:2006-11-14
  • 通讯作者: 汪世龙

Selective Apoptosis Inducing Effect of Nano-TiO2 on CHO Cells

ZHU Rong-Rong; WANG Shi-Long*; CHEN Xiao-Ping; SUN Xiao-Yu; ZHANG Rui; YAO Si-De   

  1. (School of Life Science and Technology, Tongji University, Shanghai 200092)
  • Received:2006-04-26 Revised:2006-06-06 Published:2006-11-14
  • Contact: WANG Shi-Long

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1072

被引次数

65 | 7

以人肾上皮细胞293T和中国仓鼠卵巢肿瘤细胞CHO为研究对象, 通过MTT法酶标仪检测、倒置显微镜观察和DNA ladder实验等方法, 研究了锐钛型TiO2对它们的选择性凋亡诱导作用. 实验结果表明, 纳米TiO2对肿瘤细胞具有非常明显的毒性作用, 可侵入细胞内部, 诱导CHO细胞产生细胞凋亡. 对于293T细胞, 虽然会抑制其增殖, 但不能够进入细胞膜内, 没有细胞凋亡现象的发生. 这一结论为利用纳米TiO2治疗癌症提出了一条新思路.

关键词: 纳米TiO2, CHO细胞, 293T细胞, 细胞凋亡, 细胞毒性

The diluted nano-TiO2 was used to treat 293T cells and CHO cells. 293T represents human kidney epithelium which belongs to normal cells, while CHO represents Chinese hamster ovary tumor cells. With the method of MTT assay, inverted microscope and DNA ladder experiment, the cytotoxic and apoptosis effects of nano-TiO2 on the two types of cells were studied. The results indicated that nano-TiO2 had much more severe toxic effect on CHO cells. After treatment, nano-TiO2 entered into the CHO cells and also induced cell apoptosis. These reactions did not take place in the case of 293T, although nano-TiO2 suppressed the multiplication of 293T. This finding might be significant for cancer therapy.

Key words: nano-TiO2, CHO cell, 293T cell, apoptosis, cytotoxicity