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化学学报
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化学学报 ›› 2007, Vol. 65 ›› Issue (20): 2325-2330. 上一篇    下一篇

研究论文

Langmuir-Blodgett膜超分子SM/DOPC/Chol三元脂系的原子力显微镜观察

郝长春，孙润广   

  1. (陕西师范大学物理学与信息技术学院 生物物理与生物医学工程研究室 西安 710062)
  • 投稿日期:2006-12-28 修回日期:2007-05-25 发布日期:2007-10-28
  • 通讯作者: 孙润广

Atomic Force Microscopic Observation on the Supramolecular Structure of Langmuir-Blodgett Films in the SM/DOPC/Chol Ternary System

HAO Chang-Chun； SUN Run-Guang   

  1. (Laboratory of Biophysics and Biomedical Engineering, College of Physics and Information Technology, Shaanxi Normal University, Xi'an 710062)
  • Received:2006-12-28 Revised:2007-05-25 Published:2007-10-28
  • Contact: SUN Run-Guang

PDF

1048

被引次数

27

用原子力显微镜研究了胆固醇(Chol)对鞘磷脂(SM)/1,2-二油酸甘油-3-磷脂酰胆碱(DOPC)二元脂系统结构的影响和神经酰胺对SM/DOPC/Chol三元脂系统结构的影响. 实验发现, 在SM/DOPC二元脂系统中, 胆固醇和带饱和脂肪酸链的磷脂发生相互作用形成微区结构, 随着胆固醇含量的增加, 微区的面积逐渐增大, 形成了稳定的片层结构. 当把神经酰胺加入到等摩尔配比的SM/DOPC/Chol三元脂系统中时, 随着神经酰胺比例的增加, 先形成紧密的聚集态结构, 然后逐渐演变成具有特定微区的网状结构. 研究结果表明, 微区的形成主要是由分子不同的官能团之间的相互作用所决定, 这可能在细胞信号传导等生理活动中起到重要的作用.

关键词: 脂筏, LB膜技术, 原子力显微镜

Atomic force microscopy has been used to study the effect of cholesterol (Chol) on the structure of equimolar sphingomyelin (SM)/1,2-dioleoy-sn-glycero-3-phosphocholine (DOPC) binary bilayers and ceramide on the structure of SM/DOPC/cholesterol ternary bilayers. We found that the formation of microdomain structure in the binary lipid system was attributed to the interaction between cholesterol and SM with saturated lipid chain. As the contents of cholesterol increasing, the microdomain structure gradually expanded and formed a stable lamellar structure. When adding ceramide to equimolar SM/DOPC/Chol ternary system, as the proportion of ceramide increasing, a close aggregate structure appeared at first, and then developed to a retwork structure with particular microdomain. The results showed that the formations of microdomains were mainly due to the interaction of different functional groups, which belonged to individual molecule. These microdomains may play important roles in cellular signaling.

Key words: lipid raft, LB technique, atomic force microscopy (AFM)