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化学学报
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化学学报 ›› 2007, Vol. 65 ›› Issue (21): 2382-2386. 上一篇    下一篇

研究论文

一个嫁接有羧酸羟基乙基酯的二吡啶吩嗪Ru(II)配合物的合成、与DNA的相互作用以及溶剂变色性质的研究

韩洋1，杨维春1,2，王科志*,1   

  1. (1北京师范大学化学学院 北京 100875)
    (2平顶山学院化学化工学院 平顶山 467002)
  • 投稿日期:2007-04-14 修回日期:2007-06-13 发布日期:2007-11-14
  • 通讯作者: 王科志

Study on the Synthesis, DNA Interaction, and Solvatochromism of a Hydroxyethyl Carboxylate-Grafted Dipyridophenazine Ruthenium(II) Complex

HAN Yang1; YANG Wei-Chun1,2; WANG Ke-Zhi*,1   

  1. (1 College of Chemistry, Beijing Normal University, Beijing 100875)
    (2 College of Chemistry and Chemical Engineering, Pingdingshan Univer-sity, Pingdingshan 467002)
  • Received:2007-04-14 Revised:2007-06-13 Published:2007-11-14
  • Contact: WANG Ke-Zhi

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合成并表征了一个新的Ru(II)配合物[Ru(bpy)2(hedppc)](ClO4)2 {bpy＝2,2'-联吡啶, hedppc＝二联吡啶[3,2-a: 2',3'-c]吩嗪-11-羧酸(2-羟乙基)酯}. 通过紫外-可见吸收光谱、与溴化乙锭竞争实验、粘度测量和DNA裂解实验研究了配合物与小牛胸腺DNA的相互作用性质. 结果表明配合物以插入模式与DNA键合，键合常数Kb＝(6.99±1.34)×106 mol－1•L (s＝2.03±0.04)与母体配合物[Ru(bpy)2 (dppz)]2＋相近，但光致发光和溶剂变色等光学性质与[Ru(bpy)2 (dppz)]2＋有明显的差别.

关键词: 钌(II), DNA, 紫外-可见吸收光谱, 溶剂变色

A new ruthenium(II) polypyridyl complex of [Ru(bpy)2(hedppc)](ClO4)2 {in which bpy＝2,2'-biyridine, hedppc＝2-hydroxyethyl dipyrido[3,2-a:2',3'-c]phenazine-11-carboxylate} was synthesized and characterized by elemental analysis, 1H NMR and MALDI-TOF MS. The binding of the com-plex to calf thymus (ct) DNA was studied with UV-visible absorption and luminescence spectrophotometric titrations, DNA competitive binding with ethidium bromide, viscosity measurements and thermal denatura-tion experiments. The results indicated that the complex was bound to ct-DNA through an intercala-tive mode with an intrinsic binding constant of (6.99±1.34)×106 L/mol in buffered 50 mmol/L NaCl comparable to parent [Ru(bpy)2(dppz)]2＋. The complex is per-manently non-emissive in the absence and presence of the DNA, and exhibits intriguing solvatochromic properties different from parent [Ru(bpy)2(dppz)]2＋.

Key words: ruthenium(II), DNA, UV-vis absorption spectroscopy, solvatochromism