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化学学报
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化学学报 ›› 2007, Vol. 65 ›› Issue (22): 2623-2628. 上一篇    下一篇

研究论文

MMA/MPC共聚物膜的制备及其抗凝血性能研究

张晓冬1，倪忠斌1，李英2，邱丽颖2，刘晓亚1，陈明清*,1   

  1. (1江南大学化学与材料工程学院 无锡 214122)
    (2江南大学医药学院 无锡 214122)
  • 投稿日期:2007-02-10 修回日期:2007-07-04 发布日期:2007-11-28
  • 通讯作者: 陈明清

Preparation and Blood Anticoagulation Activity of Poly(methylmetha- crylate-co-2-methacryloxyethyl phosphorylcholine) Membranes

ZHANG Xiao-Dong1; NI Zhong-Bin1; LI Ying2; QIU Li-Ying2; LIU Xiao-Ya1; CHEN Ming-Qing*1   

  1. (1 School of Chemical and Material Engineering, Jiangnan University, Wuxi 214122)
    (2 School of Medicine, Jiangnan University, Wuxi 214122)
  • Received:2007-02-10 Revised:2007-07-04 Published:2007-11-28
  • Contact: CHEN Ming-Qing

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1062

被引次数

48

采用自由基共聚制得了甲基丙烯酸甲酯与2-甲基丙烯酰氧基乙基磷酰胆碱(MPC)的共聚物, 在模板中将溶剂蒸发得到了共聚物膜. 用差示扫描量热仪(DSC)、凝胶渗透色谱(GPC)、元素分析仪(EA)、氢核磁共振(1H NMR)及扫描电子显微镜(SEM)对共聚物及其膜的结构与形态进行了表征, 并测定了膜的溶胀度与表面的亲水性, 结合对牛血清蛋白(BSA)吸附研究结果表明: 共聚物膜的溶胀度随着MPC含量的增加而逐渐上升, 并且随着温度的升高而逐渐增大; 由动态接触角(DCA)结果可知共聚物膜表面的链段可随着环境的变化而发生重排, MPC链段向膜表面的迁移提高了膜表面的亲水性, 降低了对蛋白质的吸附. 并通过体外血小板粘附试验对膜材料的抗凝血性能进行了评价. 结果表明, 当共聚物膜中w(MPC)＝0.25时, 膜表面吸附的血小板数量明显减少, 共聚物膜表现出良好的抗凝血性能.

关键词: 2-甲基丙烯酰氧基乙基磷酰胆碱, 溶胀度, 动态接触角, 牛血清蛋白, 抗凝血性

The copolymers of methyl methacrylate with 2-methacryloxyethyl phosphorylcholine (MPC) were synthesized by solution polymerization. The copolymer membranes were prepared by a solvent evaporation method and characterized by differential scanning calorimeter, gel permeation chromatography, elemental analysis, 1H nuclear magnetic resonance, and scanning electron microscopy. The equilibrium water content (EWC) and adsorption of bovine serum albumin (BSA) onto the surface of the copolymer membranes were studied, while dynamic contact angle (DCA) was used to monitor hydrophilicity of the membrane surfaces. It was found that EWC of the copolymer membranes increased with MPC mass content and system temperature. The results of DCA showed that the surface of the membranes could be mobile and able to rearrange depending on the environment in which the membranes were placed. The adsorbed amount of BSA decreased with the increment of MPC mass content in the copolymer membranes, which was able to improve the anticoagulant activity of the membranes. The blood anticoagulation of the membranes was determined by a platelet adhesion experiment. It was shown that the copolymer membranes, in which the mass content of MPC was more than 25%, appeared to be beneficial in improving the anticoagulation that was proved to suppress the platelet adhesion.

Key words: 2-methacryloxyethyl phosphorylcholine, equilibrium water content, dynamic contact angle, bovine serum albumin, blood anticoagulation