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化学学报
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化学学报 ›› 2007, Vol. 65 ›› Issue (23): 2755-2760. 上一篇    下一篇

研究论文

磁性微粒子化学发光免疫分析法测定人血清中雌三醇

吴丹凝1, 王栩1, 林金明*,1, 李振甲2, 应希堂2   

  1. (1清华大学化学系生命有机磷化学与化学生物学教育部重点实验室 北京 100084)
    (2北京科美东雅生物技术有限公司 北京科学技术研究院 北京100094)
  • 投稿日期:2007-02-27 修回日期:2007-06-14 发布日期:2007-12-14
  • 通讯作者: 林金明

Determination of Estriol in Human Serum by a Chemiluminescence Immunoassay Method Using Magnetic Microparticle

WU Dan-Ning1; WANG Xu1; LIN Jin-Ming*,1; LI Zhen-Jia2; YING Xi-Tang2   

  1. (1 The Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology of Education Ministry, Department of Chemistry, Tsinghua University, Beijing 100084)
    (2 Beijing Chemclin Biotech Co., Ltd., Beijing Academy of Science and Technology, Beijing 100094)
  • Received:2007-02-27 Revised:2007-06-14 Published:2007-12-14
  • Contact: LIN Jin-Ming

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利用化学发光免疫分析(chemiluminescence immunoassay, CLIA)高灵敏度和高特异性的特点, 将磁性微粒子应用于化学发光免疫分析中, 用两种不同的方法对人体血清内的雌三醇(E3)含量进行了测定. 磁性微粒子分别作为固相一抗包被材料和二抗分离剂参与反应. 两种方法检测雌三醇浓度的线形范围均为0.6～60 ng/mL. 其中, 固相一抗法的批内变异及批间变异系数分别小于11%和15%, 回收率为90%～116%, 健全性系数为0.9987. 二抗分离法的批内变异及批间变异系数分别小于8%和10%, 回收率为88%～118%, 健全性系数为0.9974. 两种方法分别与经典板式化学发光法对比, 检测人血清样本, 结果相关性较好, 且磁性微粒子法更为省时、简便, 适于推广应用.

关键词: 雌三醇, 磁性微粒子, 化学发光免疫分析法, 人血清

A chemiluminescence immunoassay (CLIA) method of high sensitivity, selectivity and reproducibility for the determination of estriol (E3) in human serum was developed. The magnetic microparticles were used as the second-antibody separation agent and the first-antibody solid-surface, respectively. Under the selected conditions, the two methods with magnetic microparticles as the second-antibody separation agent and the first-antibody solid-surface respectively, showed a calibration curve for E3 both in the range of 0.6～60 ng/mL. In the first-antibody method, the CVs (coefficient of variations) of intra- and inter-assays were less than 11% and 15%, respectively. The recovery was in the range of 90%～116%, and the soundness coefficient was 0.9987. In the second-antibody method, the CVs of intra- and inter-assays were less than 8% and 10%, respectively. The recovery was in the range of 88%～118%, and the soundness coefficient was 0.9974. Compared with the traditional micro-plate CLIA method, both of the proposed methods exhibited less analysis time and labor. The proposed methods have been used to determine E3 in human serum successfully.

Key words: estriol, magnetic microparticle, chemiluminescence immunoassay, human serum