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化学学报
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化学学报 ›› 2007, Vol. 65 ›› Issue (3): 177-183.    下一篇

研究通讯

含有28个氨基酸的复杂多肽的串级质谱全序列分析研究

贾韦韬1,2, 陆豪杰*,1,2, 贠栋1,2, 杨芃原*,1,2   

  1. (1复旦大学化学系 上海 200433)
    (2复旦大学生物医学研究院 上海 200032)
  • 投稿日期:2006-04-04 修回日期:2006-08-09 发布日期:2007-02-14
  • 通讯作者: 杨芃原

Sequence Analysis of a Complex Polypeptide Containing 28 Amino Acids by Tandem Mass Spectrometry

JIA Wei-Tao1,2; LU Hao-Jie*,1,2; YUN Dong1,2; YANG Peng-Yuan*,1,2   

  1. (1 Department of Chemistry, Fudan University, Shanghai 200433)
    (2 Institutes of Biomedical Sciences, Fudan University, Shanghai 200032)
  • Received:2006-04-04 Revised:2006-08-09 Published:2007-02-14
  • Contact: YANG Peng-Yuan

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被引次数

18 | 4

利用MALDI-TOF/TOF MS和ESI-MS/MS对一种含有多达28个氨基酸的复杂合成多肽成功进行了全序列测定. 通过调节激光强度、碰撞诱导解离(CID)能量等质谱参数以及依据不同序列分析软件, 获得了涵盖所有b型和y型碎片离子的串级质谱图. 结果显示这种方法可以有效地解决de novo测序方法遇到的谱峰过于复杂导致运算死机等问题. 通过讨论如何对含有超过20个氨基酸片断的多肽进行合格串级质谱实验, 为蛋白质组学肽段序列测定提供了新的方法和思路.

关键词: 串级质谱, 序列分析, 多肽

One synthetic complex polypeptide composed of 28 amino acids was successfully sequenced by matrix-assisted laser desorption/ionization-time of flight/time of flight-mass spectrometry (MALDI-TOF/ TOF MS) and electrospray ionization-tandem mass spectrometry (ESI-MS/MS). Depending on the analysis of different sequencing software and the adjustment of the mass spectrometry parameters as well as the laser intensity, the collision induced dissociation (CID) energy and so on, we obtained the perfect tandem mass spectrum which covered all the b- and y-type fragment ions. Results indicated that this method can effectively solve the difficulty occurring in de novo sequencing, such as dead-response of computer. The established method is a good supplement of peptides sequencing for proteomics research.

Key words: tandem mass spectrometry, sequence analysis, polypeptide