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化学学报
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化学学报 ›› 2007, Vol. 65 ›› Issue (9): 821-828. 上一篇    下一篇

研究论文

金纳米微粒作探针共振瑞利散射法测定某些蒽环类抗癌药物

鲁群岷, 刘忠芳, 刘绍璞*   

  1. (西南大学化学化工学院 重庆 400715)
  • 投稿日期:2006-08-02 修回日期:2006-12-31 发布日期:2007-05-14
  • 通讯作者: 刘绍璞

Resonance Rayleigh Scattering Method for the Determination of Some Anthracycline Anticancer Drugs with Gold Nanoparticle as Probe

LU Qun-Min; LIU Zhong-Fang; LIU Shao-Pu*   

  1. (School of Chemistry and Chemical Engineering, Southwest China University, Chongqing 400715)
  • Received:2006-08-02 Revised:2006-12-31 Published:2007-05-14
  • Contact: LIU Shao-Pu

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1290

被引次数

61 | 11

在近中性至弱碱性介质中, 金纳米微粒与表柔比星(EPI)、柔红霉素(DNR)和米托蒽醌(MXT)等蒽环类抗癌药物借静电引力、疏水作用力结合, 形成粒径更大的聚集体, 导致共振瑞利散射(RRS)的显著增强并产生新的RRS光谱, 三种结合产物的最大RRS峰均位于313 nm附近, 并在510～610 nm之间有一宽的散射带. 其散射强度(ΔI)与3种抗癌药物的浓度成正比, 对EPI, DNR和MXT的线性范围分别为0.009～0.50, 0.010～0.70 和0.030～1.20 μg•mL－1, 它们的检出限(3σ)分别为2.7, 3.1和9.0 ng•mL－1. 研究了反应产物的吸收、荧光和RRS光谱特征, 适宜的反应条件及分析化学性质, 发展了一种用RRS技术灵敏、简便、快速测定蒽环类抗癌药物的新方法.

关键词: 金纳米微粒, 共振瑞利散射, 蒽环类抗癌药物

In a neutral or weak alkaline medium, the gold nanoparticle can combine with an anthracycline anticancer drugs such as epirubicin, daunorubicin or mitoxantrone by the virtue of electrostatic and hydrophobic interaction, forming aggregate with bigger diameters. As a result, the resonance Rayleigh scattering (RRS) was enhanced greatly, and a new RRS spectrum appeared. The maximum RRS signals of three ion-association complexes were all located at about 313 nm, and there were another wide scattering strip between 510～610 nm. There was a linear relationship between the RRS intensity and the drug concentration in the range of 0.009～0.5 for epirubicin (EPI), 0.010～0.7 for daunorubicin (DNR) and 0.030～1.2 mg•mL－1 for mitoxantrone (MXT), respectively. Their detection limits (3σ) for three drugs were 2.7 for epirubicin, 3.1 for daunorubicin and 9.0 ng•mL－1 for mitoxantrone. In this work, the characteristics of the absorption, fluorescence and RRS spectra, the optimum conditions of the reaction and the properties of analytical chemistry were investigated. A sensitive, simple and new method for the determination of anthracycline anticancer drugs based on RRS has been developed.

Key words: gold nanoparticle, resonance Rayleigh scattering, anthracycline anticancer drug