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化学学报
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化学学报 ›› 2008, Vol. 66 ›› Issue (1): 56-62. 上一篇    下一篇

研究论文

修饰硅胶表面印迹牛血红蛋白及其识别性能的研究

刘秋叶1，李文友1，何锡文*,1，陈朗星1，张玉奎*,1,2   

  1. (1南开大学化学学院 天津 300071)
    (2中国科学院大连化学物理研究所 大连 116011)
  • 投稿日期:2007-06-14 修回日期:2007-08-17 发布日期:2008-01-14
  • 通讯作者: 何锡文

Recognition and Characterization of imprinted Bovine Hemoglobin on surface-modified silica gel

LIU Qiu-Ye1 LI Wen-You1 HE Xi-Wen*,1 CHEN Lang-Xing1 ZHANG Yu-Kui*,1,2   

  1. (1 Department of Chemistry, Nankai University, Tianjin 300071)
    (2 Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116011)
  • Received:2007-06-14 Revised:2007-08-17 Published:2008-01-14

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本文选用马来酸酐修饰后的硅胶作为载体，丙烯酰胺为功能单体，N，N’-亚甲基双丙烯酰胺为交联剂，牛血红蛋白为模板分子，采用氧化还原悬浮聚合法，合成了具有选择性识别的牛血红蛋白分子印迹聚合物。并用红外光谱(IR)、扫描电子显微镜(SEM)对聚合物进行了表征,结果表明载体表面成功接枝了分子印迹聚合物薄层。同时，选择性吸附实验表明分子印迹聚合物的具有良好的识别性能，能成功的实现水溶液中牛血红蛋白的富集。

关键词: 表面修饰分子印迹聚合物, 牛血红蛋白, 硅胶修饰, 氧化还原聚合

In this article, the silca modified with maleic anhydride was synthesized as carrier material. Then, Molecularly imprinted polymers (MIP) based on surface-modified silica gel were prepared by redox polymerization of acrylamide and N, N’-methylene bisacrylamide as the functional monomer and the crosslinker in the present of bovine hemoglobin as template molecule. The results of IR and scanning electron microscope (SEM) demonstrated the formation of a grafting polymer layer on the modified silica gel surface. The adsorption studies also showed the imprinted polymers had good adsorption capacity and specific recognition for bovine hemoglobin in aqueous solution.

Key words: Surface-modified molecularly imprinted polymer, Bovine hemoglobin, grafting of silica gel, redox polymerization