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化学学报
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化学学报 ›› 2008, Vol. 66 ›› Issue (10): 1221-1227. 上一篇    下一篇

研究论文

细胞色素b5突变体P40V血红素微环境核磁共振研究

谌章舟1,2，王志强3，吴厚铭2，黄仲贤3，伍晓雄1，曹春阳*,2   

  1. (1华中农业大学 武汉 430070)
    (2中科院上海有机化学研究所 生命有机国家重点实验室 上海 200032)
    (3复旦大学化学系 上海 200433)
  • 投稿日期:2007-12-31 修回日期:2008-03-10 发布日期:2008-05-28
  • 通讯作者: 曹春阳

1H NMR Studies On Heme Microenvironment of Cytochrome b5 Mutant P40V

SHENG, Zhang-Zhou1,2, WANG, Zhi-Qiang3, WU, Hou-Ming2, HUANG, Zhong-Xian3 WU, Xiao-Xiong1, CAO, Chun-Yang*,2   

  1. (1 Huazhong Agricultural University, Wuhan 430070)
    (2 State Key Laboratory of Bio-organic and Natural Product Chemistry, Shanghai Institute of Organic Chemistry, Chinese Acadmey of Sciences, Shanghai 200032)
    (3 Department of Chemistry, Fudan University, Shanghai 200433)
  • Received:2007-12-31 Revised:2008-03-10 Published:2008-05-28
  • Contact: CAO, Chun-Yang

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832

被引次数

2

细胞色素b5是一个具有多种重要生理功能的低自旋金属蛋白, 作为呼吸链系统的主要电子传递体之一受到了广泛的重视. 为了研究细胞色素b5表面疏水斑区Pro40在细胞色素b5结构与功能上的重要性, 本文利用定点突变技术把细胞色素b5中Pro40突变为Val40, 并采用1D与2D 1H NMR的方法对其进行分析, 探讨了Pro40突变为Val40对细胞色素b5血红素微环境的影响.

关键词: 细胞色素b5, P40V, 血红素, NMR

As a main component of respiratory chain in living system, cytochrome b5 is a low spin electron-transfer hemo protein that carries out a variety of important physiological functions and has been extensively studied by biochemical and biophysical methods. To probe the structural and functional importance of the residue Pro40 in the hydrophobic patch of cytochrome b5 surface, this paper studied its mutant P40V by using modern 1D and 2D 1H NMR techniques to examine its heme environment changes resulting from the mutation from P40 to V40.

Key words: cytochrome b5, P40V, heme, NMR