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化学学报
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化学学报 ›› 2008, Vol. 66 ›› Issue (3): 355-361. 上一篇    下一篇

研究论文

低频超声波照射下卟啉锰(HP-Mn)配合物对牛血清白蛋白(BSA)损伤的研究

王晓芳1，王君*,1,2，张朝红3，熊大珍2，陈立江1，徐锐2，张向东2   

  1. 辽宁大学化学学院 辽宁大学 辽宁大学环境科学系 辽宁大学化学系
  • 投稿日期:2007-06-08 修回日期:2007-09-13 发布日期:2008-02-14
  • 通讯作者: 王君

Investigation on damage of bovine serum albumin (BSA) by porphyrin-Mn (HP-Mn) complex under low frequency ultrasonic irradiation

WANG Xiao-Fang1; WANG Jun*,1,2 ;ZHANG Zhao-Hong3 ;XIONG Da-Zhen2; CHEN Li-Jiang1; XU Rui2 ZHANG Xiang-Dong2   

  1. (1 Department of Pharmacy, Liaoning University, Shenyang 110036)
    (2 Department of Chemistry, Liaoning University, Shenyang 110036)
    (3 Department of Environmental Science, Liaoning University, Shenyang 110036)
  • Received:2007-06-08 Revised:2007-09-13 Published:2008-02-14
  • Contact: WANG Jun

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应用紫外-可见(UV-vis)光谱和荧光光谱研究了卟啉-锰(HP-Mn)配合物与牛血清白蛋白(BSA)的相互作用及在超声波作用下HP-Mn对BSA的损伤作用，并探讨了超声波照射时间，HP-Mn浓度，酸度，离子种类和强度等因素对BSA损伤的影响。结果表明，HP-Mn对BSA荧光的猝灭属于静态猝灭，两者主要通过静电作用相互结合，同时也存在着配位作用，作用距离(r)为3.49 nm。另外，在超声波作用下，HP-Mn能够明显损伤BSA。损伤程度随着超声波照射时间的增长，酸度的升高和HP-Mn浓度的增大而增大，但离子种类和强度的影响较为复杂。这一结果为声动力学(SDT)疗法治疗肿瘤应用于临床具有重要的意义。

关键词: 超声, 卟啉锰(HP-Mn)配合物, 牛血清白蛋白(BSA), 损伤

The interaction between hematopotphyrin-Mn (HP-Mn) complex and bovine serum albumin (BSA) as well as the damage of BSA in the presence of HP-Mn complex under ultrasonic irradiation were studied by UV-vis and fluorescence spectra. In addition, the influence of ultrasonic irradiation time, HP-Mn concentration, solution acidity (pH), ionic kind and strength were also detected. The results showed that the quenching of fluorescence of BSA caused by HP-Mn complex belonged to the static quenching. HP-Mn complex and BSA interact each other mainly through electrostatic and coordinate actions and the binding distance (r) is 3.49 nm. Otherwise, under ultrasonic irradiation the BSA molecules were obviously damaged by HP-Mn complex. The damage degree rose up with the increase of ultrasonic irradiation time, solution acidity (pH) and HP-Mn concentration, while the influence of ionic kind and strength were complicated. The research results are of great significance for driving sonodynamic treatment (SDT) to treat tumour in clinic application.

Key words: ultrasound, hematoporphyrin-Mn (HP-Mn) complex, bovine serum albumin (BSA), damage