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化学学报
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化学学报 ›› 2008, Vol. 66 ›› Issue (5): 581-586. 上一篇    下一篇

研究论文

水溶性有机物与细胞膜的结合作用

李璐，吴玲玲，郜洪文*，潘琦，李硕   

  1. (同济大学环境科学与工程学院 污染控制与资源化研究国家重点实验室 上海 200092)
  • 投稿日期:2007-06-07 修回日期:2007-08-27 发布日期:2008-03-14
  • 通讯作者: 郜洪文

Binding of Water-soluble Organic Compounds to Cellular Membrane

LI Lu, WU Ling-Ling, GAO Hong-Wen*, PAN Qi LI Shuo   

  1. (State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai 200092)
  • Received:2007-06-07 Revised:2007-08-27 Published:2008-03-14
  • Contact: GAO Hong-Wen

PDF

750

被引次数

11 | 1

以二氨基蓝3R (DAB)和玫瑰红B (RB)两种离子型有机物为探针, 研究了其与体外单层磷脂膜(SPM)和大肠杆菌之间的相互作用, 结合作用符合Langmuir和Temkin等温化学吸附方程. 考察了pH、离子强度和温度对吸附作用的影响, 分析了DAB, RB与SPM和细胞的结合形式、结合键和结合位置. 结果表明, DAB, RB与SPM和细胞外膜有强烈的单分子层化学吸附, 是自发的放热反应, 主要通过电荷对吸引、氢键等非共价键结合在SPM和细胞外表面, 且绝大部分DAB, RB积累、滞留在细胞膜壁上. 混合作用时, 两种有机物存在竞争吸附结合现象.

关键词: 离子型有机物, 单层磷脂膜, 细胞膜, 大肠杆菌, 化学吸附

The binding of ionic organic compounds, anionic diamine blue 3R (DAB) and cationic rhodamine B (RB) to in vitro single phospholipids membrane (SPM) and Escherichia coli (E. coli) strains was investigated. The interactions correspond to the Langmuir and Temkin adsorption isotherm in only monolayers. The influence of ionic strength, pH and temperature on the adsorption were examined and analyzed. The binding force, type and interaction sites of DAB and RB were determined and identified. The interaction of DAB and RB with SPM and the cells are exothermal and idiopathic, which contained the union of non-covalent bonds, such as electrostatic bond, hydrogen bond and van der Waals force. Moreover, most of DAB and RB were accumulated on the excellular membrane and walls, to hardly enter the cytoplasm. Besides, the adsorption competition was found when both DAB and RB existed simultaneously.

Key words: ionic organic compound, single phospholipid membrane, cellular membrane, E. coli, chemical adsorption