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化学学报
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化学学报 ›› 2009, Vol. 0 ›› Issue (1): 54-58. 上一篇    下一篇

研究论文

超声合成Fe3O4@SiO2复合纳米磁性粒子用于质粒DNA的提纯

王显祥1,王森杨婉身单志3   

  • 投稿日期:2008-07-07 修回日期:2008-07-28 发布日期:2009-12-30
  • 通讯作者: 王显祥 E-mail:xianxiangwang@hotmail.com
  • 基金资助:
    四川省教育厅自然科学科研重点项目（2005A033）、四川农业大学引进人才基金（007202）

Ultrasonic Synthesis of Fe3O4@SiO2 Nano-composite Magnetic Particles for Plasmid DNA Purification

  • Received:2008-07-07 Revised:2008-07-28 Published:2009-12-30

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1997

被引次数

23

用超声合成了用于质粒DNA提取的Fe3O4@ SiO2复合纳米磁性粒子。用透射电镜（TEM）、红外光谱（FT-TR）、震动样品磁场计（VSM）、X光电子能谱仪(XPS)等方法对合成的复合磁性粒子的表面形貌、结构、磁性质等进行了表征，合成的复合磁微粒粒径分布均匀，在15-20nm，磁响应性好。用该复合磁微粒提取的DNA纯度能达到A260/A280=1.8±0.1，琼脂糖电泳证明质粒DNA结构基本没有被破坏，主要为超螺旋结构，可满足PCR等后续分子生物学操作的要求。

关键词: 复合纳米磁性粒子, 超声, Fe3O4@SiO2, DNA, 提取

Fe3O4@SiO2 core-shell nanoparticles for plasmid DNA purification were prepared by ultrasound. The prepared nano-composite particles were investigated by transmission electron microscopy (TEM), infrared spectrum (FT-IR), vibrating sample magnetometer (VSM) and X photoelectron energy spectrum (XPS) in order to elucidate the morphology, magnetic and other properties of the nano-composites. The results showed that their uniform distribution of particle size about 20 nm and good magnetic responsiveness. Using the magnetic composite nanoparticles to extract plasmid DNA, the A260/A280 rations of DNA samples were 1.8±0.1. DNA structure was superhelix, not damaged with agarose gel electrophoresis confirm. The plasmid DNA could be used for PCR and other molecular biology operation.

Key words: Nano-composite magnetic particles, Ultrasonic, Fe3O4@SiO2, DNA, Purification