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化学学报
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化学学报 ›› 2009, Vol. 67 ›› Issue (10): 1123-1126. 上一篇    下一篇

研究论文

牛蒡苷元的生物转化及电喷雾质谱研究

赵宇峰a,b 宋凤瑞b 赵立平a 刘淑莹*,b

  

  1. (a上海交通大学系统生物医学研究院 上海 200240)
    (b中国科学院长春应用化学研究所长春质谱中心 长春 130022)

  • 投稿日期:2008-04-01 修回日期:2008-12-08 发布日期:2009-05-28
  • 通讯作者: 刘淑莹

Studies on the Biotransformation of Arctigenin Using Electrospray Ionization Mass Spectrometry

Zhao, Yufeng a,b Song, Fengrui b Zhao, Liping a Liu, Shuying *,b   

  1. (a Shanghai Center for Systems Biomedicine, Shanghai Jiao Tong University, Shanghai 200240)
    (b Changchun Center of Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022)
  • Received:2008-04-01 Revised:2008-12-08 Published:2009-05-28
  • Contact: Liu, Shuying

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被引次数

20 | 4

采用牛蒡苷元和人肠内细菌真杆菌sp. ARC-2 (Eubacterium sp. ARC-2)体外温孵的方法, 探讨牛蒡苷元的生物转化机理. 木脂素类化合物在ESI负离子模式条件下形成准分子离子[M－H]－, 利用离子阱和傅立叶变换离子回旋共振电喷雾串联质谱方法直接分析牛蒡苷元的转化产物. 研究结果表明, 在真杆菌sp. ARC-2的作用下, 牛蒡苷元经过3次脱甲基反应最终生成4’,4’’-二羟基肠内酯.

关键词: 牛蒡苷元, 真杆菌sp. ARC-2, 生物转化, 电喷雾质谱, 4’,4’’-二羟基肠内酯

To study the biotransformation of arctigenin, arctigenin was anaerobically incubated with Eubacterium sp. ARC-2 of human intestinal bacteria in vitro. Arctigenin formed a molecular ion [M－H]－ in negative ion mode. The arctigenin and its metabolites were investigated directly by the electrospray ionization tandem mass spectrometry ion trap and Fourier transform ion cyclotron resonance. Arctigenin was transformed to 4’,4’’-dihydroxylenterolactone by E sp. ARC-2 through 3 types of demethylation products.

Key words: arctigenin, Eubacterium sp. ARC-2, biotransformation, electrospray ionization mass spectrometry, 4’,4’’-dihydroxylenterolactone