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化学学报
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化学学报 ›› 2009, Vol. 67 ›› Issue (16): 1869-1874. 上一篇    下一篇

研究论文

电喷雾电离质谱法研究环肽对HIV-1调控区DNA的识别及其相关碎裂机理和稳定性

李卉卉a,b 郑 波a 叶蕴华a 袁 谷*,a

  

  1. (a北京大学化学与分子工程学院化学生物学系 北京分子科学国家实验室
    生物有机与分子工程教育部重点实验室 北京 100871)
    (b南京师范大学化学与环境科学学院 江苏省生物功能材料重点实验室 南京 210097)

  • 投稿日期:2009-03-10 修回日期:2009-04-01 发布日期:2009-08-28
  • 通讯作者: 袁谷

DNA-Recognition of Cyclic Peptides in Regulatory Region of HIV-1 Gene by Electrospray Ionization Mass Spectrometry

Li, Huihui a,b Zheng, Bo a Ye, Yunhua a Yuan, Gu *,a   

  1. (a Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of the Ministry of Education, Department of Chemical Biology, College of Chemistry, Peking University, Beijing 100871)
    (b Jiangsu Key Laboratory of Biofunctional Materials, School of Chemistry and Environmental Science,
    Nanjing Normal University, Nanjing 210097)
  • Received:2009-03-10 Revised:2009-04-01 Published:2009-08-28
  • Contact: Yuan, Gu

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被引次数

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利用电喷雾电离质谱(ESI-MS)和二级质谱(MS/MS)研究了六种结构不同的环五肽, 环七肽以及环十肽与HIV-1调控区DNA的非共价键相互作用. 在研究中比较了不同识别分子与靶序列DNA结合的强弱, 发现环七肽CP5对靶点DNA具有高亲合性的结合. 用MS/MS法研究了环肽与DNA复合物的碎裂机理; 用升温实验研究了其热稳定性, 发现与CP5结合后能提高HIV-1双螺旋DNA的热稳定性.

关键词: HIV-1基因, ESI-质谱法, DNA识别, 环肽

Electrospray ionization mass spectrometry (ESI-MS) was utilized to investigate the recognition of six cyclopeptides (cyclopentapeptides, cycloheptapeptides and cyclodecapeptide) in the regulatory region of HIV-1 gene. It was found that cyclic peptide CP5 had the higher binding affinity with the HIV-1 DNA, and ESI MS/MS data revealed that the CP5 stabilized the target duplex DNA.

Key words: HIV-1 gene, ESI-MS, DNA-recognition, cyclic peptide