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化学学报
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化学学报 ›› 2009, Vol. 67 ›› Issue (7): 705-708. 上一篇    

研究简报

NRSF/REST蛋白结构域锌指9核磁共振溶液结构确定

居家莹a,b 杨中正b 吴厚铭b 王仲孚a 曹春阳*,b

  

  1. (a西北大学 西安 710069)
    (b中国科学院上海有机化学研究所生命有机化学国家重点实验室 上海 200032)

  • 投稿日期:2008-07-03 修回日期:2008-11-06 发布日期:2009-04-14
  • 通讯作者: 曹春阳

Solution Structure Determination of Zinc Finger 9 in NRSF/REST by NMR

Ju, Jiaying a,b Yang, Zhongzheng b Wu, Houming b
Wang, Zhongfu a Cao, Chunyang *,b
  

  1. (a Northwest University, Xi’an 710069)
    (b State Key Laboratory of Bio-organic and Natural Product Chemistry,
    Shanghai Institute of Organic Chemistry, CAS, Shanghai 200032)
  • Received:2008-07-03 Revised:2008-11-06 Published:2009-04-14
  • Contact: Cao, Chunyang

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NRSF/REST蛋白是神经元特异性基因表达的负调控因子. 其C端抑制结构域的C2H2型锌指结构域与共抑制蛋白CoREST相互作用能部分诱导NRSF/REST蛋白的负调控功能, 但具体的分子机制不清楚. 为了深入地研究该机制,利用核磁共振方法确定了该结构域C2H2锌指的溶液三维结构, 结果表明它具有典型的Cys2His2锌指三维结构, 揭示该结构域在NRSF/REST蛋白与DNA或RNA特异性识别上可能具有某些关键作用.

关键词: NRSF/REST, 锌指9, 核磁共振, 溶液结构

NRSF/REST is a kind of negative transcription factor for some special neuron gene expression. The interaction between the zinc finger 9 located in its C-terminal repression domain and co-repression factor CoREST partially induces NRSF/REST negative transcription function. However the detailed mechanism remains unclear. In order to study this mechanism later, here the solution structure of zinc finger 9 was determined by NMR techniques, which finally demonstrated that it contained classic C2H2 zinc finger properties. This might indicate its key function in the special identification of DNA or RNA by NRSF/REST.

Key words: NRSF/REST, zinc finger 9, NMR, solution structure