化学学报 ›› 2009, Vol. 67 ›› Issue (9): 1008-1012. 上一篇    下一篇

研究论文

酶蛋白直接修复嘧啶二聚体机理的模型研究

季学保a 汤文建b 宋钦华*,a

  

  1. (a中国科学技术大学化学系 合肥 230026)
    (b安徽医科大学药学院 合肥 230032)

  • 投稿日期:2008-07-25 修回日期:2008-12-05 发布日期:2009-05-14
  • 通讯作者: 宋钦华

Model Studies of the Repair Mechanism for Cyclobutane Pyrimidine Dimer by Apoenzyme

Ji, Xuebao a Tang, Wenjian b Song, Qinhua *,a   

  1. (a Department of Chemistry, University of Science and Technology of China, Hefei 230026)
    (b School of Pharmacy, Anhui Medical University, Hefei 230032)
  • Received:2008-07-25 Revised:2008-12-05 Published:2009-05-14
  • Contact: Song, Qinhua

去辅基的DNA光解酶在280 nm光辐照下, 能高效修复底物嘧啶二聚体(Φ=0.56). 为了模拟酶蛋白的这一修复过程, 合成了色氨酸(Trp)和/或酪氨酸(Tyr)与胸腺嘧啶二聚体(D)共价连接的化合物, 作为酶-底物复合物的模型, 研究了它们在295 nm光照射下氨基酸残基光敏化二聚体裂解的性质, 测定了二聚体裂解量子产率(Φ), 获得一些新的结果并对其进行了分析.

关键词: DNA光复活, 环丁烷胸腺嘧啶二聚体, 色氨酸, 酪氨酸, 模型研究

The apoenzyme of DNA photolyase can efficiently repair its substrate after excitation by 280 nm light (Φ=0.56). In order to mimic this repair process of apoenzyme, we synthesized two model compounds, in which tryptophan and/or tyrosine was covalently linked to cyclobutane thymine dimer, and measured the splitting quantum yield of dimer under 295 nm light. By investigating the photosensitized splitting properties of the dimer, some new insights into the intramolecular electron-transfer process were gained.

Key words: DNA photoreactivation, cyclobutane thymine dimer, tryptophan, tyrosine, model study