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化学学报
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化学学报 ›› 2010, Vol. 68 ›› Issue (06): 508-514. 上一篇    下一篇

研究论文

三齿多吡啶钴(II)配合物的合成、表征及其与DNA的相互作用研究

杨浩*,1，刘建超2，包晓玉1，行文茹1，刘珊珊1，陈文涛1，邱东方1   

  1. (1南阳师范学院化学系 南阳 473061)
    (2三门峡职业技术学院 三门峡 472000)
  • 投稿日期:2009-07-17 修回日期:2009-09-17 发布日期:2010-03-28
  • 通讯作者: 杨浩 E-mail:yanghaony@yahoo.com.cn

Synthesis, Characterization and DNA Interaction of Tridentate Polypyridyl Cobalt(II) Complexes

Yang Hao*,1 Liu Jianchao2 Bao Xiaoyu1 Xing Wenru1 Liu Shanshan1 Chen Wentao1 Qiu Dongfang1   

  1. (1 Department of Chemistry, Nanyang Normal University, Nanyang 473061)
    (2 Sanmenxia Polytechnic College, Sanmenxia 472000)
  • Received:2009-07-17 Revised:2009-09-17 Published:2010-03-28

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合成了两种三齿多吡啶钴(II)配合物 (A)和[Co(H2Bzimpy)2]Cl2 (B), 用元素分析、IR对配合物的组成和结构进行了表征, 测定了配合物A的晶体结构. 用电子吸收光谱、荧光光谱、循环伏安法及凝胶电泳实验等方法研究了配合物与DNA的相互作用. 结果表明配合物A和B与小牛胸腺(CTDNA)的作用属部分插入和静电结合, 凝胶电泳实验表明配合物A在310 nm光辐射15 min, 可使超螺旋pBR322DNA断裂为开环缺口型和线型DNA.

关键词: 三齿多吡啶配体, 钴(II)配合物, 晶体结构, DNA, 光裂解

Two tridentate polypyridyl complexes of cobalt(II) [DMPhTPY＝6,6 -dimethyl-4 -phenyl-2,2 :6 ,2 -terpyridine] (A) and [Co(H2Bzimpy)2]Cl2 [H2Bzimpy]2＝2,6-bis(benz- imidazol-2-yl)pyridine] (B) were synthesized and characterized by elemental analysis and IR. The crystal structure of complex A was determined. The interactions of the complexes with calf thymus DNA (CTDNA) have been investigated by absorption spectroscopy, fluorescence spectroscopy, cyclic voltammetry and agarose gel electrophoresis. The studies suggested that complexes A and B bind to CTDNA by partial intercalation and electrostatic interaction. The agarose gel electrophoresis showed that complex A converted supercoiled pBR322 DNA to nicked and linear DNA when it was irradiated at 310 nm for 15 min.

Key words: tridentate polypyridyl ligand, cobalt(II) complex, crystal structure, DNA, photocleavage

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