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化学学报
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化学学报 ›› 2010, Vol. 68 ›› Issue (10): 1032-1036. 上一篇    

研究简报

肝素诱导人细胞型朊蛋白构象变化的光谱表征

肖赛金，胡萍萍，谭克俊，甄淑君，黄承志   

  1. (西南大学发光与实时分析教育部重点实验室 重庆 400715)
  • 投稿日期:2009-09-08 修回日期:2009-12-11 发布日期:2010-01-19
  • 通讯作者: 肖赛金 E-mail:xiaosj@swu.edu.cn
  • 基金资助:

    国家重大科学基础研究计划

Spectra Characterization of the Conformational Changes of Human Cellular Prion Protein Induced by Heparin

XIAO Sai-Jin, HU Ping-Ping, TAN Ke-Dun, JUAN Chu-Jun, HUANG Cheng-Zhi   

  1. (Education Ministry Key Laboratory on Luminescence and Real-Time Analysis, Southwest University, Chongqing 400715)
  • Received:2009-09-08 Revised:2009-12-11 Published:2010-01-19
  • Contact: Saijin Xiao E-mail:xiaosj@swu.edu.cn

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粘多糖在朊病毒病中所发挥的作用目前仍存在争议. 以肝素钠作为粘多糖的代表, 通过共振光散射光谱、荧光光谱和圆二色光谱的变化研究了肝素钠与人重组细胞型朊蛋白(rhPrPC23-231)的相互作用. 结果表明, 肝素钠与朊蛋白相互作用后光散射和荧光信号均得到增强, 并且使朊蛋白的荧光寿命有一定程度的延长. 圆二色光谱表明肝素钠能诱导朊蛋白从富含α-螺旋的构象向富含β-折叠的构象转变.

关键词: 朊病毒病, 朊蛋白, 粘多糖, 肝素钠

The role of glycosaminoglycans (GAGs) played in prions is still in controversy. By using heparin as an example, we investigated the interaction of GAGs and recombinant human cellular prion protein (rhPrPC23-231) by measuring the spectral features of the resonance light scattering (RLS), fluorescence and circular dichroism (CD). It was found that the intensity of both RLS and fluorescence get increased when the interaction of heparin with rhPrPC23-231 occurred, with the extension of the fluorescence lifetime. Meanwhile, the CD spectra indicate that heparin could induce the conformation rich in alfa-helix change to conformation rich in beta-sheet.

Key words: prion, prion protein, glycosaminoglycans