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化学学报
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化学学报 ›› 2010, Vol. 68 ›› Issue (11): 1148-1152. 上一篇    

研究简报

十二烷基硫酸钠诱导Rhodobacter azotoformans外周捕光复合体LH2 B800细菌叶绿素特异性解离

董彦敏1，崔小华1，杨素萍*,1,2，岳慧英2，赵春贵*,1,2，焦念志3，曲音波4   

  1. (1山西大学化学生物学与分子工程教育部重点实验室 生命科学与技术学院 太原 030006)
    (2华侨大学生物工程与技术系 厦门 361021)
    (3厦门大学近海海洋环境科学国家重点实验室 厦门361005)
    (4山东大学微生物技术国家重点实验室 济南 250100)
  • 投稿日期:2009-08-04 修回日期:2009-11-18 发布日期:2010-02-03
  • 通讯作者: 杨素萍 E-mail:yangsuping@sxu.edu.cn; yangsuping@hqu.edu.cn
  • 基金资助:

    国家自然科学基金(30470044)、国家科技基础条件平台建设项目(2005DKA21209)、近海海洋环境科学国家重点实验室（厦门大学）高级访问学者基金(MELRS0907)、山西省回国留学人员科研资助项目(200713)资助项目

Specific Release of Bacteriochlorophylls B800 of LH2 from Rhodobacter azotoformans Induced by Sodium Dodecyl Sulfate

Dong Yanmin1 Cui Xiaohua1 Yang Suping*,1,2 Yue Huiying2, Zhao Chungui*,1,2 Jiao Nianzhi3 Qu Yinbo4   

  1. (1 Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, School of Life Science and Technology, Shanxi University, Taiyuan 030006)
    (2 Department of Bioengineering and Biotechnology, Huaqiao University, Xiamen 361021)
    (3 State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen 361005)
    (4 State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100)
  • Received:2009-08-04 Revised:2009-11-18 Published:2010-02-03

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采用吸收光谱法研究了十二烷基硫酸钠(SDS)诱导Rhodobacter azotoformans外周捕光复合体LH2 细菌叶绿素(bacteriochlorophylls, BChls)的解离行为和规律. 结果表明: 室温下, 在10 mmol•L－1 Tris-HCl (pH 8.0)缓冲液中, 低浓度SDS只诱导LH2中B800 细菌叶绿素解离生成游离BChls, 而B850不受影响; 当浓度达到0.08% (w/V) 时, 能特异性地诱导B800缺失, 其缺失过程和游离BChls的生成过程均符合单指数模型, 且二者的速率常数近似相等. 高浓度SDS能同时诱导B800和B850解离生成游离BChls, 其中B800可发生缺失, 而B850则不完全解离, 解离过程均符合单指数模型; B800对SDS更敏感, 其解离速率常数约是B850的4倍, 游离BChls生成速率常数明显低于B800解离速率常数, 而与B850解离速率常数相接近.

关键词: LH2, SDS, 细菌叶绿素, Rhodobacter azotoformans

The release behaviors of bacteriochlorophyll (BChl) molecules from the LH2 complex of Rhodobacter azotoformans induced by sodium dodecyl sulfate (SDS) were investigated using absorption spectroscopy. The results indicated that BChl-B800 molecules (B800) are released from their binding sites and transformed into free BChl by incubating LH2 sample in 10 mmol·L-1 Tris-HCl (pH 8.0) buffer containing SDS of low concentration at room temperature. However, the BChl-B850 molecules (B850) aren’t affected. The dynamics of B800 release and free BChl formation induced by 0.08% (w/v) SDS can be well fitted by the monoexponential model. The rate constant of B800 release is nearly equal to that of free BChl formation. The release of both B800 and B850 of LH2 complexes can be induced by high concentration SDS, simultaneously. B800 can be completely transformed into free BChl, but not for B850. Although both of their release processes show monoexponential models in 1% SDS solution, the release rate constant of B850 is remarkably lower than that of B800 and close to that of free BChl formation.

Key words: LH2, SDS, bacteriochlorophylls, Rhodobacter azotoformans