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化学学报
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化学学报 ›› 2010, Vol. 68 ›› Issue (14): 1343-1348.    下一篇

研究论文

1,4-二羟基蒽醌及其Y3＋配位聚合物与DNA相互作用研究

殷卫峰，欧植泽*，高云燕*，郝平，郭创龙，王中丽   

  1. (西北工业大学理学院应用化学系 西安 710072)
  • 投稿日期:2010-04-04 修回日期:2010-05-25 发布日期:2010-06-12
  • 通讯作者: 欧植泽 E-mail:ouzhize@nwpu.edu.cn
  • 基金资助:

    竹红菌素衍生物与血浆蛋白质作用的构效关系研究;环糊精包结作用对功能化富勒烯C60光化学性质的影响;碳纳米材料/核黄素的能量传递和电子转移反应调控研究;功能化富勒烯的簇集行为对其光化学性质的影响

Interaction of 1,4-Dihydroxyanthraquinone and Its Yttrium Coordination Polymer with DNA

YIN Wei-Feng, OU Zhi-Ze , GAO Yun-Yan-*, HAO Ping, GUO Chuang-Long, WANG Zhong-Li   

  1. (Department of Applied Chemistry, School of Science, Northwestern Polytechnical University, Xi an 710072)
  • Received:2010-04-04 Revised:2010-05-25 Published:2010-06-12
  • Contact: Ou Zhize E-mail:ouzhize@nwpu.edu.cn

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利用紫外-可见吸收光谱(UV-Vis)、核磁共振(1H NMR)、红外光谱(FTIR)研究了1,4-二羟基蒽醌(DHA)与Y3＋的配位作用, 结果表明Y3＋与DHA能形成物质的量之比为1∶1的一维链状配位聚合物Y-DHA. 与DHA相比, Y-DHA在可见光区的吸收大幅增强, 同时具有良好的水溶性．紫外-可见吸收光谱、荧光光谱及DNA熔链温度实验研究结果表明, Y-DHA与CT DNA可通过静电作用和沟槽结合的方式结合, 而DHA与CT DNA的作用方式主要为沟槽结合. 循环伏安法表明, Y-DHA的还原电位(－0.324 V vs SCE)要高于DHA的还原电位(－0.387 V vs SCE). 无氧条件下, Y-DHA光损伤DNA的能力要明显高于DHA.

关键词: 蒽醌, 配位聚合物, DNA光损伤, 光诱导电子转移

The interaction of 1,4-dihydroxyanthraquinone (DHA) with Y3＋ was studied by UV-Vis, 1H NMR, and FTIR. Y3＋ and DHA can form one-dimensional chain coordination polymer Y-DHA with a 1∶1 stoichiometry. Y-DHA possesses better water solubility and shows stronger absorption in visible region than DHA. The interaction of Y-DHA and DHA with CT DNA was studied by UV-Vis, fluorescence spectra, and DNA melting temperature experiments. The results indicate that the groove binding and electrostatic interaction are the major modes for the association of Y-DHA with CT DNA, while groove binding is the mainly mode for the interaction of DHA with CT DNA. Voltammetry studies demonstrate that the reduction potential of Y-DHA (－0.324 V vs SCE) is more positive than that of DHA (－0.387 V vs SCE). Under anaerobic conditions, Y-DHA shows stronger DNA photocleavage ability than DHA.

Key words: anthraquinone, coordination polymer, DNA photocleavage, photo-induced electron transfer