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化学学报
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化学学报 ›› 2010, Vol. 68 ›› Issue (17): 1776-1780. 上一篇    

研究简报

气相色谱-质谱法检测番茄红素酱中甲苯、间二甲苯

姚洁，张家琪，陈彤，王公应   

  1. (中国科学院成都有机化学研究所 成都 610041)
  • 投稿日期:2009-12-15 修回日期:2010-03-31 发布日期:2010-04-09
  • 通讯作者: 陈彤 E-mail:chentongw@sina.com.cn

Detection of Toluene and m-Xylene in Lycopene Sauce by GC-MS

YAO Jie, ZHANG Jia-Qi, CHEN Tong, WANG Gong-Ying   

  1. (Chengdu Institute of Organic Chemistry, Chinese Academy of Sciences, Chengdu 610041)
  • Received:2009-12-15 Revised:2010-03-31 Published:2010-04-09

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1379

被引次数

5

建立了气相色谱-质谱法(GC-MS)检测番茄红素酱中甲苯、间二甲苯的分析方法, 考察了三种样品前处理方法对甲苯、间二甲苯的检测效果. 结果表明, 高温下番茄红素会重排降解生成甲苯、间二甲苯, 从而使甲苯、间二甲苯检测值偏高. 用乙醇萃取后, 顶空吹扫冷凝法检测, 能最大程度避免高温的影响, 是对番茄红素酱中甲苯、间二甲苯准确定量合适的前处理方法. 在选定的条件下, 甲苯和间二甲苯分别在1～100 μg/mL, 3～300 μg/mL的范围内线性关系良好, 方法回收率甲苯在92.2%～98.0%之间, 间二甲苯在90.3%～97.4%之间; 相对标准偏差(RSD)均小于10.0%; 检测限(LOD)为0.03 μg/g.

关键词: 番茄红素酱, 甲苯, 间二甲苯, 气相色谱-质谱

The method for the determination of the content of toluene and m-xylene in lycopene sauce by gas chromatography mass spectrometry (GC­MS) was established. The effects of three different sample pretreatment methods were tested. It was found that lycopene could undergo rearrangement and degradation to toluene and m-xylene at high temperature so that their test values were significantly higher than their authentic values. Extraction with ethanol followed by headspace-blowing-condensation proved to be an effective sample pretreatment method that could avoid the adverse high temperature effect and thus enable accurate determination of the content of toluene and m-xylene in lycopene sauce. Following this method, good linear relationships in the range of 1～100 µg/mL for toluene and in the range of 3～300 µg/mL for m-xylene were obtained, accompanied by recovery of 92.2%～98.0% for toluene and 90.3%～97.4% for m-xylene, of which the relative standard deviations were both less than 10.0% and the limits of determination were both 0.03 µg/g.

Key words: lycopene sauce, toluene, m-xylene, GC-MS