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化学学报
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化学学报 ›› 2010, Vol. 68 ›› Issue (9): 839-844. 上一篇    下一篇

研究论文

丁二酸二异辛酯磺酸钠(AOT)/Triton X-100混合反胶束体系中纤维素酶降解纤维素的研究

杨淑娟，孔长青，张可，毕红艳，李晶，张洪林*   

  1. (曲阜师范大学化学与化工学院 曲阜 273165)
  • 投稿日期:2009-07-16 修回日期:2009-10-26 发布日期:2009-12-21
  • 通讯作者: 张洪林 E-mail:zhanghl@qfnu.edu.cn
  • 基金资助:

    山东省自然科学基金（No. Y2006B13）

Study on Enzymatic Degradation of Cellulose in Diisooctyl Succinate Sodium Sulfonate (AOT)/Triton X-100 Mixed Reverse Micellar System

Yang Shujuan Kong Changqing Zhang Ke Bi Hongyan Li Jing Zhang Honglin   

  1. (College of Chemical and Chemical Engineering, Qufu Normal University, Qufu 273165)
  • Received:2009-07-16 Revised:2009-10-26 Published:2009-12-21

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1406

被引次数

27 | 3

对于底物不溶于水的纤维素降解反应而言, 为了增强纤维素酶的活性, 在丁二酸二异辛酯磺酸钠(AOT)/异辛烷反胶束体系中加入非离子表面活性剂Triton X-100进行纤维素降解实验. 结果表明, 在AOT中加入非离子表面活性剂Triton X-100可以使纤维素酶的活性提高, 非离子表面活性剂Triton X-100与AOT的最佳物质的量之比是0.20. 考察了水与表面活性剂的物质的量之比(<I>W</I><sub>o</sub>)、不同酸度(pH)和不同温度(<I>T</I>)等其他反应条件对纤维素降解反应的影响. 研究结果表明, 反应的最佳条件是: <I>W</I><sub>o</sub>为3.3, <I>T</I>为315.11 K, pH为5.10.

关键词: 混合反胶束, 热动力学, 对比进度法, 微量热法

To enhance the cellulase activity for the reaction of water-insoluble substrates, the diisooctyl succinate sodium sulfonate (AOT)/isooctane reverse micellar was modified by co-adsorption of Triton X-100. An appreciable increase of cellulase activity was observed and the results show that the optimal molar fraction of AOT/Triton X-100 is 0.20. The influences of other reaction conditions on the molar ratio of water to total surfactants (<I>W</I><sub>o</sub>), different pH and temperature have been investigated, and the optimal conditions for the reaction were <I>W</I><sub>o</sub>＝3.3, <I>T</I>＝315.11 K, pH＝5.10.

Key words: mixed reverse micelles, thermokinetic, reduced extent method, microcalorimetry

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