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化学学报
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化学学报 ›› 2011, Vol. 69 ›› Issue (13): 1570-1574. 上一篇    下一篇

研究论文

中药黄芩中神经氨酸酶抑制剂的超滤质谱筛选研究

刘舒1,2, 邢俊鹏1, 闫峻3, 宋凤瑞1, 刘志强*,1, 刘淑莹1   

  1. (1中国科学院长春应用化学研究所 长春质谱中心 长春 130022)
    (2中国科学院研究生院 北京 100039)
    (3吉林大学药学院 长春 130021)
  • 投稿日期:2011-01-01 修回日期:2011-03-01 发布日期:2011-03-29
  • 通讯作者: 刘志强 E-mail:liuzq@ciac.jl.cn
  • 基金资助:

    国家科技部创新方法研究专项

Screening and Structures Characterization of Neuraminidase Inhibitors from Radix Scutellaria Extract by Ultrafiltration LC-MSn

Liu Shu1,2; Xing Junpeng1; Yan Jun3; Song Fengrui1; Liu Zhiqiang*,1; Liu Shuying1   

  1. (1 Changchun Center of Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022)
    (2 Graduate School of the Chinese Academy of Sciences, Beijing 100039)
    (3 School of Pharmacy, Jilin University, Changchun 130021)
  • Received:2011-01-01 Revised:2011-03-01 Published:2011-03-29

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1844

被引次数

18

采用超滤质谱分析技术, 结合体外酶活性实验方法, 从传统中药黄芩提取物中筛选神经氨酸酶抑制剂. 研究结果表明, 中药黄芩提取物具有较强的神经氨酸酶抑制活性, 利用超滤质谱方法从中筛选并鉴定出了六种具有潜在神经氨酸酶抑制活性的化合物, 为开发神经氨酸酶抑制剂提供了实验依据.

关键词: 超滤质谱, 黄芩提取物, 神经氨酸酶抑制剂

In vitro neuraminidase inhibition assays and ultrafiltration liquid chromatography with diode- array detector coupled to electrospray ionization tandem mass spectrometry (LC-DAD-ESI-MSn) were combined to screening neuraminidase inhibitors from Radix Scutellaria extract. As a result, Radix Scutellaria extract showed strong neuraminidase inhibitory activity, six compounds presented neuraminidase inhibitory effects were observed and identified by LC-DAD-ESI-MSn.

Key words: ultrafiltration LC-DAD-ESI-MSn, Radix Scutellaria extract, neuraminidase inhibitor