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化学学报
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化学学报 ›› 1991, Vol. 49 ›› Issue (1): 94-97. 上一篇    下一篇

研究论文

藻胆蛋白生物合成的模型反应I. 藻蓝胆素硫醚键的形成

易国良;蒋丽金;马金石   

  1. 中国科学院感光化学研究所.北京(100101)
  • 发布日期:1991-01-15

A model reaction of biosynthesis of phycobiliproteins I. Thioether formation of phycocyanobilin

Yi Guoliang;Jiang Lijin;Ma Jinshi   

  1. Inst Photog Chem, Acad Sinica.Beijing(100101)
  • Published:1991-01-15

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藻蓝胆素二甲酯2a在氢氧化铵的催化作用下可与巯基乙醇发生加成反应,通过UV-Vis,MS和^1HNMR鉴定了加成物的结构,巯基乙醇是加成在A环的3'-C上,得到3-氢-3'-[β-羟基乙硫基]藻蓝胆素二甲酯3,产率为80%。不仅模拟了藻蓝胆素的硫醚键形成,也进一步证明了它是藻蓝蛋白生物合成的最后一步。利用^1HNMR技术对加成物的构型进行了详细的讨论。

关键词: 硫醚键, 藻蓝胆素, 模型反应, 生物合成, 藻胆蛋白, 巯基乙醇, 氢氧化铵

The addition reaction of phycocyanobilin dimethyl ester with 2- mercaptoethanol could occur in ammoniacal methanolic solution and gave 80% yield of 3-hydro-3'-[β -hydroxy-ethylthio)-phycocyanobilin dimethyl ester which was identified by UV-Vis, MS and ^1H NMR spectra. The reaction was considered to be a model reaction for biosynthesis of phycocyanin. The configuration of the adduct was studied.

Key words: PHYCOCYANOBILIN, BIOSYNTHESIS, MERCAPTOETHANOL, AMMONIUM HYDROXIDE

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