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化学学报
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化学学报 ›› 2008, Vol. 66 ›› Issue (21): 2353-2359. 上一篇    下一篇

研究论文

[Cr(III)(4-ASA)(en)2]Cl配合物的光化学活性

刘 斌 孙占国 杨斌盛*

  

  1. (山西大学分子科学研究所化学生物学与分子工程教育部重点实验室 太原 030006)

  • 投稿日期:2007-12-12 修回日期:2008-04-29 发布日期:2008-11-14
  • 通讯作者: 杨斌盛

Photochemical Activities of Chromium(III) Complex [Cr(III)(4-ASA)(en)2]Cl

LIU, Bin SUN, Zhan-Guo YANG, Bin-Sheng*   

  1. (Key Laboratory of Chemical Biology and Molecular Engineering, Ministry of Education,
    Institute of Molecular Science, Shanxi University, Taiyuan 030006)
  • Received:2007-12-12 Revised:2008-04-29 Published:2008-11-14
  • Contact: YANG, Bin-Sheng

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被引次数

1

通过吸收光谱、荧光光谱、电导率和ESI-MS质谱等方法讨论了铬配合物[Cr(III)(4-ASA)(en)2]Cl (4-ASA: 4-aminosalicylic acid dianion, en: ethylenediamine)在不同温度、不同pH溶液中的稳定性及光化学稳定性. 实验表明, 该配合物的溶液(pH 7.4)在日光照射下发生了光化学取代反应, 取代产物为[Cr(4-ASA)(en)(H2O)2]＋. 同时研究了配合物及其光照产物对EDTA的动力学反应和对DNA的切割反应. 琼脂糖凝胶电泳实验表明, 配合物的光化学产物[Cr(4-ASA)(en)(H2O)2]＋能有效切割pBR 322 DNA.

关键词: 铬(III)配合物, 光化学, DNA切割

The stability, photochemistry activities and kinetic reaction of chromium complex [Cr(III)(4-ASA)- (en)2]Cl•2H2O (4-ASA: 4-aminosalicylic acid dianion, en: ethylenediamine) were discussed by means of UV-visible, fluorescence spectra, conductivity and electrospray ionization mass spectrometry. The complex is sensitive to light. The photochemistry product was deduced to be [Cr(4-ASA)(en)(H2O)2]＋, and then the Gel electrophoresis of plasmid DNA shows that the photochemistry product can promote the cleavage of pBR 322 DNA efficiently.

Key words: chromium(III) complex, photochemistry, DNA damage