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化学学报
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化学学报 ›› 2006, Vol. 64 ›› Issue (18): 1933-1936. 上一篇    下一篇

研究简报

镓转铁蛋白与HL60细胞的结合

李英奇, 刘斌, 赵春贵, 杨斌盛*   

  1. (山西大学分子科学研究所 化学生物学与分子工程教育部重点实验室 太原 030006)
  • 投稿日期:2005-10-17 修回日期:2006-03-07 发布日期:2006-09-28
  • 通讯作者: 杨斌盛

Binding of Gallium-Containing Transferrin to HL60 Cells

LI Ying-Qi; LIU Bin; ZHAO Chun-Gui; YANG Bin-Sheng*   

  1. (Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Molecular Science, Shanxi University, Taiyuan 030006)
  • Received:2005-10-17 Revised:2006-03-07 Published:2006-09-28
  • Contact: YANG Bin-Sheng

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被引次数

6 | 1

用125I示踪法测定了不同形式的镓转铁蛋白(GaC-Tf, Ga2-Tf, GaC-Tf-FeN)与HL60细胞的结合作用. 结果表明0 ℃、pH＝7.4时GaC-Tf, Ga2-Tf和GaC-Tf-FeN均与细胞膜结合, 表观结合常数分别为(1.2±0.14)×108, (1.1±0.19)×108和(1.8±0.42)×108 L/mol; 同时测定了125I标记的脱铁转铁蛋白(apoTf)与HL60细胞的表观结合常数为(0.97±0.20)×108 L/mol; HL60细胞的平均受体数为(4.8±0.11)×105. 通过体外研究表明, 不同形式的镓转铁蛋白与受体的结合能力次序为apoTf≈GaC-Tf≈Ga2-Tf＜GaC-Tf-FeN.

关键词: 转铁蛋白, 镓(Ⅲ), 125 I示踪法, HL60细胞

The interaction of 125I-labelled various species of transferrin including GaC-Tf, Ga2-Tf and GaC-Tf-FeN with HL60 cells was investigated. The results showed that the proteins bound to membrane of cells at pH＝7.4 and 0 ℃. The association constants were (1.2±0.14)×108, (1.1±0.19)×108 and (1.8±0.42)×108 L/mol for GaC-Tf, Ga2-Tf and GaC-Tf-FeN, respectively. At the same time, the association constant for 125I-labelled apotransferrin was (0.97±0.20)×108 L/mol. There were about (4.8±0.11)×105 transferrin receptors per cell surface. In vitro studies indicated that com-plexes of Ga and transferrin bind to transferrin receptor with the order of apoTf≈GaC-Tf≈Ga2-Tf＜GaC-Tf-FeN in terms of their association constants.

Key words: transferrin, gallium(III), 125I-labeling, HL60 cells