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化学学报
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化学学报 ›› 2002, Vol. 60 ›› Issue (3): 504-508. 上一篇    下一篇

研究论文

大孔聚甲基丙烯酸缩水甘油酯固定化酶载体的合成及性能研究

乌云高娃;卢冠忠;郭杨龙;王筠松;魏东芝   

  1. 华东理工大学工业催化研究所.上海(200237);生物反应器国家重点实验室.上 海(200237)
  • 发布日期:2002-03-15

The Studies on Synthesis and Properties of Glycidyl Methacrylate-N, N'-methylene-bis (acrylamide) Copolymer Supports of Enzyme Catalyst

Wuyun Gaowa;Lu Guanzhong;Guo Yanglong;Wang Yunsong;Wei Dongzhi   

  1. Research Institute of Industrial Catalysis East China University of Science and Technology.Shanghai(200237);East China University of Science and Technology.Shanghai(200237)
  • Published:2002-03-15

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684

被引次数

56 | 15

选用反应性单体甲基丙烯酸缩水甘油酯，以N,N'-亚甲基双（丙烯酰胺）为交 联剂，甲酰胺为致孔剂，用反相悬浮聚合方法制得一系列珠状共聚物载体，通过测 定其表面的物化性能、表面的环氧基浓度和固定青霉素酰化酶活性，讨论了交联剂 用量、配比及合成条件对共聚物表面性能和固定化青霉素酰化酶活性的影响。

关键词: 甲基丙烯酸酯, 缩水甘油酯, 丙烯酰胺, 悬浮聚合, 青霉素酶

Reactive, macroporous and beaded glycidyl methacrylate-N, N'- methylene-bis (acrylamide) copolymers of widely varying crosslink densities were synthesized with formamide as progenic agent by inverse suspension copolymerization. The effects of crosslink density on structure properties and apparent activity of immobilized penicillin G acylase were investigated.Specific surface area increased, pore volume, porosity and average pore diameter decreased with concomitant increase in crosslinking monomer. Immobilized enzyme activity of 254.8 U/g was obtained with a polymer with ω = 60% crosslinking having a mean pore diameter of 4.9 nm and an oxirane group content of 0.005 mmol·m~(-2).

Key words: METHYLPROPENOIC ACID ESTER, GLYCIDYL ESTER, PROPENAMIDE, SUSPENSION POLYMERIZATION, PENICILLINASE

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