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化学学报
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化学学报 ›› 2002, Vol. 60 ›› Issue (7): 1269-1273. 上一篇    下一篇

研究论文

血红蛋白在磷脂-月桂酸修饰的玻碳电极上的电化学行为及其分析应用

金松子;王韬;张春煦;沈含熙   

  1. 南开大学化学学院.天津(300071)
  • 发布日期:2002-07-15

Electrochemical Behavior of Hemoglobin at a Lipid-Lauric Acid Modified GC Electrode and Its Application to the Determination of Proteins

Jin Songzi;Wang Tao;Zhang Chunxu;Shen Hanxi   

  1. Chemical School, Nankai University.Tianjin(300071)
  • Published:2002-07-15

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607

被引次数

59 | 7

报道了血红蛋白（Hb）在磷脂-月桂酸修饰的玻碳电极上的电化学行为，在+0. 8～-0.7V (vs. Ag/AgCl)电位范围内，于pH6.0的0.01mol/L的KH_2PO_4- Na_2HPO_4底液中，血红蛋白产生不可逆的还原电流峰。还原峰电流与血红蛋白浓 度在1.25 * 10~(-8)～4.31 * 10~(-7) mol/L范围内呈良好线性关系。该电极可作 为检测血红蛋白的新型的高灵敏度电化学生物传感器。

关键词: 血红蛋白, 类脂膜, 磷脂, 月桂酸, 玻碳电极, 化学修饰电极

The electrochemical behavior of hemoglobin at a lipid-lauric acid modified glassy carbon electrode (GC) is reported. In the potential range of +0.8～-0.7 V (vs. Ag/AgCl), an irreversible cathodic peak was observed at -0.4 V at the scan rate of 20 mV/s. In the medium of 0.01 mol/L KH_2PO_4-Na_2HPO_4 (pH 6.0), the cathodic peak current shows linear relationship with the concentration of hemoglobin in the range of 1.25 * 10~(-8)～4.31 * 10~(-7) mol/L. The electrode can be used as an approach for determining hemoglobin with high sensitivity.

Key words: HEMOGLOBIN, LIPID MEMBRANE, PHOSPHOLIPID, LAURIC ACID, GLASSY CARBON ELECTRODE, CHEMICAL MODIFIED ELECTRODE

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