关键词: 毛细管电泳, 手性拆分, 山莨菪碱, 固相萃取

To establish a capillary electrophoresis method under which anisodamine enantiomers can be sufficiently separated for quantification, ,. Hydroxypropylhydroxypropyl-β-cyclodextrin (HP-β-CD), methyl-β- cyclodextrin (Me-β-CD) and carboxymethyl-β- cyclodextrin (CM-β-CD) were used as chiral selectors. Effects of the concentration of cyclodextrin, pH and concentration of buffer were investigated. The baseline separation of 4 four enantiomers of anisodamine was obtained in 110 mmol/L Tris-H3PO4 buffer-20.0 mg/mL HP-β-CD-5.0 mg/mL CM-β-CD at pH 4.0. Prior to electrophoresis, the analytes were purified and preconcentrated by solid phase extraction from plasma. Standard curves constructed using blank plasma spiked with anisodamine in the ranges 77.86～0.39 µg/mL, showed acceptable linearity with correlation coefficients, r≥0.999. The detection limits for the four4 enantiomers of anisodamine were all 0.08 µg/mL. Mean intra- and inter-day precisions (RSD% ) waswere＜4.2% and 6.5 %, respectively. The method showed recoveries for the 4 four enantiomers ranging from 95.1% to 105%. Acceptable precision and accuracy of the method was were demonstrated for pharmacokinefic studies by determining the concentrations of anisodamine enantiomers in rabbit plasma, after administration of 75 mg of anisodamine during 3 three consecutive days.

Key words: capillary electrophoresis, chiral separation, anisodamine, solid phase extraction