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化学学报
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化学学报 ›› 2007, Vol. 65 ›› Issue (16): 1543-1547. 上一篇    下一篇

研究通讯

电喷雾质谱法研究聚酰胺、酰亚胺衍生物与HIV-1基因DNA的相互作用

李卉卉, 韩德伟, 袁谷*   

  1. (北京大学化学学院化学生物学系 北京分子科学国家实验室 生物有机与分子工程教育部重点实验室 北京 100871)
  • 投稿日期:2007-03-30 修回日期:2007-05-20 发布日期:2007-08-28
  • 通讯作者: 袁谷

Investigation of Interaction of HIV-1 DNA and Polyamides, Imide Derivatives by Electrospray Ionization Mass Spectrometry

LI Hui-Hui; HAN De-Wei; YUAN Gu*   

  1. (Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic and Molecular Engineering of Education Ministry, College of Chemistry, Department of Chemical Biology, Peking University, Beijing 100871)
  • Received:2007-03-30 Revised:2007-05-20 Published:2007-08-28
  • Contact: YUAN Gu

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803

被引次数

5

利用电喷雾质谱(ESI-MS)研究了6种识别分子与HIV-1基因调控区DNA的非共价键相互作用. 在研究中比较了不同识别分子与靶序列DNA结合的强弱, 发现双萘酰亚胺衍生物4对此序列DNA具有高亲合性的结合, 并分析了识别分子与DNA复合物的碎裂机理以及结合模式.

关键词: HIV-1基因, ESI-质谱法, DNA识别分子, 寡聚酰胺, 酰亚胺衍生物

Electrospray ionization mass spectrometry was utilized to investigate the non-covalent interaction of HIV-1 gene and six DNA-recognizing molecules. It revealed that imide derivative 4, a potent binder of HIV-1 DNA, had the higher binding affinity with the duplex DNA among six DNA-recognizing molecules.

Key words: HIV-1 gene, ESI-MS, DNA-recognizing molecule, polyamide, imide derivative