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化学学报
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化学学报 ›› 2007, Vol. 65 ›› Issue (20): 2303-2308. 上一篇    下一篇

研究论文

Au掺杂Fe3O4纳米粒子酶传感器的制备及其应用于有机磷农药检测的研究

闵红，曲云鹤，李晓华，谢宗红，卫银银，金利通   

  1. (华东师范大学化学系 上海 200062)
  • 投稿日期:2006-12-30 修回日期:2007-04-03 发布日期:2007-10-28
  • 通讯作者: 金利通

Au-doped Fe3O4 Nanoparticle Immobilized Acetylcholinesterase Sensor for the Detection of Organophosphorus Pesticide

MIN Hong QU Yun-He LI Xiao-Hua XIE Zong-Hong WEI Yin-Yin JIN Li-Tong   

  1. (Department of Chemistry, East China Normal University, Shanghai 200062)
  • Received:2006-12-30 Revised:2007-04-03 Published:2007-10-28
  • Contact: JIN Li-Tong

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合成了金掺杂的四氧化三铁纳米粒子(Au-Fe3O4), 以壳聚糖为交联剂, 制备了电流型乙酰胆碱酯酶(AChE)生物传感器, 并将其应用于有机磷农药(OPs)的检测. 实验表明, Au-Fe3O4纳米粒子具有良好的生物兼容性, 能够有效地促进电子传递, 修饰了Au-Fe3O4纳米粒子的酶传感器, 响应速度快, 检测灵敏度高, 稳定性好; 固定在传感器上的乙酰胆碱酯酶有良好的酶动力学响应, 其表观米氏常数( )为10.3 mmol/L. 利用有机磷农药对乙酰胆碱酯酶的抑制作用, 以硫代乙酰胆碱(ATCh)为底物, 对有机磷农药敌敌畏进行了检测, 检测限达到4.0×10－13 mol/L.

关键词: Au-Fe3O4, 乙酰胆碱酯酶, 有机磷农药, 敌敌畏

Au-doped magnetic Fe3O4 nanoparticles were synthesized and used for fabrication of acetylcholinesterase (AChE) immobilized electrochemical sensor for the detection of organophosphorus pesticides (OP), such as dichlorvos. The degree of inhibition of the AChE enzyme by OP compounds was determined by measuring the electrooxidation current of the thiocholine generated by the AChE catalyzed hydrolysis of acetylthiocholine (ATCh). The use of these mixed nanoparticles was shown to provide both significant enzyme stabilization and low detection limit for the biosensor. A value of 10.3 mmol/L for the apparent Michaelis-Menten constant ( ) was obtained. The biosensor detected as low as 4.0×10－13 mol/L dichlorvos.

Key words: Au-Fe3O4, acetylcholinesterase, organophosphorus pesticide, dichlorvos