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化学学报
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化学学报 ›› 2009, Vol. 67 ›› Issue (20): 2368-2374. 上一篇    下一篇

研究论文

吹扫/捕集-热脱附气质联用法对荷叶挥发油成分的对比分析

张赟彬*,a   缪存铅a,b   崔俭杰a,b   

  1. (a上海应用技术学院香料香精技术与工程学院 上海 200235) (b上海应用技术学院上海香料研究所 上海 200232)
  • 投稿日期:2008-12-17 修回日期:2009-03-26 发布日期:2009-06-01
  • 通讯作者: 张赟彬 E-mail:qingkong0204@163.com
  • 基金资助:

    上海市教委重点项目（07ZZ165）

Comparative Analysis of Volatile oils form Lotus Leaves by Purge and Trap-Thermal Desorption Gas Chromatography-Mass Spectrometry

Zhang, Yunbin*,a    Miao, Cunqian a,b    Cui, Jianjie a,b   

  1. (a School of Perfume and Aroma Technology, Shanghai Institute of Technology, Shanghai 200235) (b Shanghai Research Institution of Fragrance and Flavor Industry, Shanghai Institute of Technology, Shanghai 200232)
  • Received:2008-12-17 Revised:2009-03-26 Published:2009-06-01

PDF

2365

被引次数

29

采用同时蒸馏萃取提取得到荷叶挥发油, 通过吹扫/捕集-热脱附法(P&T-TD)对上述提取物中挥发性成分进行富集, 以气质联用(GC/MS)进行定性检测, 同时与直接进样GC/MS法分析的成分进行比较. 两种方法成功分离分析出有机酸、酯、醛、醇、酚、烷烃、芳香烃、烯烃以及含氮、硫、氧杂原子的化合物等共计84种成分, 其中P&T-TD GC/MS鉴定出63种有机化合物, GC/MS鉴定出41种有机化合物, 有20种成分共同检出. 对比分析表明: P&T-TD GC/MS的吹扫/捕集-热脱附过程能富集各种组分, 相比GC/MS分析, 可以鉴定出微量成分及更多挥发性和半挥发性成分, 在精油等挥发性成分的分析检测中使用优势明显.

关键词: 吹扫/捕集-热脱附, 气质联用, 荷叶, 挥发油

Volatile oils were extracted from lotus leaves using simultaneous distillation extraction (SDE). Volatile components in lotus-leaf extract were enriched through a purge and trap-thermal desorption (P&T-TD) method, and analyzed qualitatively with gas chromatography-mass spectrometry (GC/MS), which were comparative by analyzed with the method of direct injection GC/MS simultaneously. Totally 84 organic compounds were successfully separated and checked out with the two methods, including the organic acids, esters, aldehydes, alcohols, phenols, alkanes, aromatic hydrocarbons, olefins, and other compounds with miscellaneous atoms of nitrogen, sulfur, or oxygen, in which 63 compounds were checked out with P&T-TD GC/MS, while 41 compounds with direct injection GC/MS, and 20 compounds in common with the two methods. Comparison analysis showed that the process of P&T-TD could enrich analyzed components so P&T-TD GC/MS could check out minimal components or more volatile/semi-volatile components. P&T-TD GC/MS is a more suitable method to analyze volatile components, such as essential oils.

Key words: purge and trap-thermal desorption, gas chromatography-mass spectrometry, lotus leaf, volatile oil

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