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化学学报
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化学学报 ›› 2008, Vol. 66 ›› Issue (9): 1059-1066. 上一篇    下一篇

研究论文

五味子中木脂素类成分的高效液相色谱-电喷雾质谱研究

黄鑫1,2，宋凤瑞1，刘志强1，刘淑莹*,1   

  1. (1中国科学院长春应用化学研究所 长春质谱中心 长春 130022)
    (2中国科学院研究生院 北京 100039)
  • 投稿日期:2007-08-30 修回日期:2007-12-07 发布日期:2008-05-14
  • 通讯作者: 刘淑莹

Studies on the Lignans in Extract of the Fruits of Schisandra chinensis and Schisandra sphenanthera by High Performance Liquid Chromatography-Electrospray Ionization Mass Spectrometry

HUANG Xin12 SONG Feng-Rui1 LIU Zhi-Qiang1 LIU Shu-Ying*1   

  1. (1 Changchun Center of Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022)
    (2 Graduate School of the Chinese Academy of Sciences, Beijing 100039)
  • Received:2007-08-30 Revised:2007-12-07 Published:2008-05-14
  • Contact: LIU Shu-Ying

PDF

1579

被引次数

87

采用液相色谱-电喷雾质谱联用(HPLC-ESI-MSn)技术, 对北五味子与南五味子中木脂素类成分进行了系统研究. 通过HPLC-ESI-MS技术, 获得了相应化合物的保留时间、紫外光谱和分子量等信息, 利用电喷雾多级串联质谱技术(ESI-MSn), 获得了相应化合物的结构信息. 研究结果表明, 北五味子与南五味子的主要木脂素成分除5个共有成分外其它成分差异较大, 并且其共有成分含量差别较大. 在此基础上, 建立了简便、快速的北五味子与南五味子药材分析鉴定的新方法.

关键词: 北五味子, 南五味子, 木脂素, 高效液相色谱-电喷雾质谱

High performance liquid chromatography-electrospray ionization mass spectrometry (HPLC- ESI-MSn) has been applied to identify and differentiate the lignan constituents of the fruits of Schisandra chinensis and Schisandra sphenanthera. The data of retention time, UV spectra and molar masses were obtained. The ESI-MSn data of the [M＋H]＋ ions provide detailed structural information on the lignans. The major lignan constituents in the fruits of Schisandra chinensis and Schisandra sphenanthera were identified. Their difference was found in the special components and the contents of five common components. The experimental results indicated that Schisandra chinensis and Schisandra sphenanthera could be differentiated easily, rapidly and effectively by the HPLC-ESI-MSn method.

Key words: Schisandra chinensis, Schisandra sphenanthera, lignan, HPLC-ESI-MSn