SIOC English
化学学报
高级检索

化学学报 ›› 2006, Vol. 64 ›› Issue (23): 2344-2350. 上一篇    下一篇

研究论文

抗氧化剂的毛细管电泳-间接化学发光联用在线评价方法研究

郑国灿1, 陈华1,2, 陈志涛1, 甘婷婷2, 冯波1, 夏之宁*,1,2   

  1. (1重庆大学化学化工学院 重庆 400044)
    (2重庆大学生物工程学院 重庆 400044)
  • 投稿日期:2006-01-26 修回日期:2006-06-20 发布日期:2006-12-14
  • 通讯作者: 夏之宁

Study on the On-line Evaluation Method for Antioxidants by Capillary Electrophoresis with Indirect Chemiluminescence Detection

ZHENG Guo-Can1; CHEN Hua1,2; CHEN Zhi-Tao1; GAN Ting-Ting2; FENG Bo1; XIA Zhi-Ning*,1,2   

  1. (1 College of Chemistry and Chemical Engineering, Chongqing University, Chongqing 400044)
    (2 College of Bioengineering, Chongqing University, Chongqing 400044)
  • Received:2006-01-26 Revised:2006-06-20 Published:2006-12-14
  • Contact: XIA Zhi-Ning

PDF

812

被引次数

23

根据碱性条件下羟自由基与luminol反应会产生化学发光而抗氧化剂能够清除羟自由基从而抑制发光的原理, 结合毛细管电泳技术, 建立了一种针对多组分共存体系中抗氧化组分的在线评价的新方法. 对这种毛细管电泳-间接化学发光检测技术, 优化化学发光的各种条件, 考察了抗氧化剂硫脲和麻黄碱的抗氧化活性, 采用曲线拟合求出它们对羟自由基的半数清除浓度(IC50), 得出抗氧化活性大小为麻黄碱＞硫脲, 这与荧光分光光度法的结果一致. 该方法初步应用于评价中药槐米提取物化学组分的抗氧化活性.

关键词: 毛细管电泳, 化学发光, 抗氧化剂, 评价, 在线

A novel method of on-line evaluation of antioxidants by capillary electrophoresis (CE) with indirect chemiluminescence (CL) detection was reported. It is based on the CL of luminol suppressed for lessened hydroxy radical which is scavenged by antioxidants in base condition. Optimal chemiluminescence conditions were obtained, and the antioxidant activities of thiourea and ephedrine were evaluated. The IC50 values of thiourea and ephedrine were obtained by fitting curves, and the order of antioxidant activity is ephedrine＞thiourea. The result was consistent with that of fluorospectrophotometry. The proposed method was preliminarily applied to evaluating the antioxidant activity of components of Sophora japonica L.

Key words: capillary electrophoresis, chemiluminescence, antioxidant, evaluation, on-line