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化学学报
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化学学报 ›› 2006, Vol. 64 ›› Issue (12): 1253-1259. 上一篇    下一篇

研究论文

胆酸盐在酸性介质中自聚集作用的共振瑞利散射光谱及其分析应用研究

南海军，刘忠芳，刘绍璞*   

  1. (西南大学化学化工学院 重庆 400715)
  • 投稿日期:2005-07-15 修回日期:2006-02-19 发布日期:2006-06-28
  • 通讯作者: 刘绍璞

Resonance Rayleigh Scattering Spectra of Self-aggregation for Bile Salts in Acidic Media and Their Analytical Application

NAN Hai-Jun, LIU Zhong-Fang, LIU Shao-Pu*   

  1. (School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715)
  • Received:2005-07-15 Revised:2006-02-19 Published:2006-06-28
  • Contact: LIU Shao-Pu

PDF

818

被引次数

46

研究了牛磺胆酸钠、甘牛胆酸钠、胆酸钠和脱氧胆酸钠等四种胆酸盐在酸性介质中的聚集作用对共振瑞利散射光谱的影响及其分析应用. 结果表明: 在一定浓度的HCl, H2SO4或HNO3溶液中, 四种胆酸盐均能自聚集形成粒径增大的聚集体. 该聚集体能导致溶液RRS显著增强, 并产生了新的RRS光谱. 不同胆酸盐在同种介质中的反应产物具有相似的光谱特征, 最大RRS波长分别位于349 (HNO3介质), 359 (H2SO4介质)和369 nm (HCl介质). 在一定范围内, 胆酸盐的浓度与散射强度(ΔIRRS)成正比. 对于不同的体系其检出限在12.0～21.8 ng/mL之间. 方法灵敏度高, 选择性较好, 而且十分简便快速. 可用于市售蛇胆川贝液和血清样品中胆酸盐的测定.

关键词: 牛磺胆酸钠, 甘牛胆酸钠, 胆酸钠, 脱氧胆酸钠, 共振瑞利散射光谱

In an acid medium of HCl, H2SO4 or HNO3, bile salts, such as sodium taurocholate, sodium tauroglycocholate, sodium deoxycholate and cow sodium cholate, were able to self-aggregate to form large particles to produce new enhanced resonance Rayleigh scattering (RRS) signals. The RRS peaks were similar in the same medium for the four reaction products. But their spectral characteristics were a little different in the three media. The maximum RRS peak was at 349 (HNO3), 359 (H2SO4) and 369 nm (HCl), respectively, while the intensity of ΔIRRS was directly proportional to the concentration of bile salt in a certain concentration range. Their detection limits for the different kinds of bile salt systems are between 12.0～21.8 ng/mL. It was shown that this method has not only high sensitivity and good selectivity, but also simplicity and rapidity. It was applicable to the determination of the commercial bile salts and serum samples with satisfactory results. In addition, the reaction mechanism and the reasons for the enhancement of RRS were discussed.

Key words: sodium taurocholate, sodium tauroglycocholate, sodium deoxycholate, sodium cholate, resonance Rayleigh scattering spectrum