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化学学报
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化学学报 ›› 2006, Vol. 64 ›› Issue (20): 2115-2119. 上一篇    下一篇

研究论文

甲基百里酚蓝-钐(III)配合物与鲱鱼精DNA的相互作用

王兴明*,1, 黎泓波1, 刘海萍1, 胡亚敏1, 丁立生2, 赵仕林3   

  1. (1西南科技大学材料科学与工程学院化学系 绵阳 621010)
    (2中国科学院成都生物研究所 成都 610041)
    (3四川师范大学化学学院 成都 610066)
  • 投稿日期:2005-12-22 修回日期:2006-04-20 发布日期:2006-10-28
  • 通讯作者: 王兴明

Interaction between Sm(III)(MTB)2 Metal Complex and Herring Sperm DNA

WANG Xing-Ming*,1; LI Hong-Bo1; LIU Hai-Ping1; HU Ya-Min1; DING Li-Sheng2; ZHAO Shi-Lin3   

  1. (1 Department of Chemistry, Southwest University of Science and Technology, Mianyang 621010)
    (2 Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041)
    (3 College of Chemistry, Sichuan Normal University, Chengdu 610066)
  • Received:2005-12-22 Revised:2006-04-20 Published:2006-10-28
  • Contact: WANG Xing-Ming

PDF

958

被引次数

25

在pH＝7.25的Tris-HCl缓冲溶液中, 以中性红(NR)作为光谱探针, 采用UV光谱、FS光谱、粘度等方法研究了甲基百里酚蓝(MTB)与稀土金属离子钐[Sm(III)]形成的配合物Sm(III)(MTB)2与鲱鱼精DNA的作用机制. 确定了Sm(III)(MTB)2与鲱鱼精DNA之间有嵌插作用方式存在, 说明Sm(III)(MTB)2金属配合物能使鲱鱼精DNA的功能产生一定影响.

关键词: 甲基百里酚蓝-钐(III)配合物, 中性红, 鲱鱼精DNA

The mechanism of interaction between Sm(III)(MTB)2 [methylthymol blue-Sm(III)] metal complex and herring sperm DNA has been studied by using neutral red (NR) as a spectral probe with the ways of UV-Vis spectra, fluorescence spectra and viscometric method in buffer solution of Tris-HCl (pH＝7.25). It was confirmed that intercalation binding was the mode of interaction between Sm(III)(MTB)2 complex and herring sperm DNA. The results indicate that the functions of herring sperm DNA were changed with the interaction between Sm(III)(MTB)2 rare earth metal complex and herring sperm DNA.

Key words: methylthymol blue-Sm(III) complex, neutral red, herring sperm DNA