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化学学报
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化学学报 ›› 2005, Vol. 63 ›› Issue (11): 1003-1007. 上一篇    下一篇

研究论文

一种可逆键合电泳微芯片的制作及在蛋白质分离中的应用

庄贵生1，刘菁1，贾春平1，金庆辉1，赵建龙*1 2，王惠民3   

  1. (1中国科学院上海微系统与信息技术研究所 上海 200050)
    (2生物芯片上海国家工程研究中心 上海 201203)
    (3南通大学附属医院 南通 226001)
  • 投稿日期:2004-07-14 修回日期:2005-02-21 发布日期:2010-12-10
  • 通讯作者: 赵建龙

Manufacture of Reversible Electrophoresis Chip and Its Application to Protein Detection

ZHUANG Gui-Sheng1, LIU Jing1, JIA Chun-Ping1, JIN Qing-Hui1, ZHAO Jian-Long*1,2, WANG Hui-Min3   

  1. (1 Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050)
    (2 National Engineering Center for Biochip at Shanghai, Shanghai 201203)
    (3 Affiliated Hospital of Nantong University, Nantong 226001)
  • Received:2004-07-14 Revised:2005-02-21 Published:2010-12-10
  • Contact: ZHAO Jian-Long

PDF

1274

被引次数

36

阐述了一种可逆键合电泳微芯片的制作方法, 以及电泳微芯片在蛋白质分离、临床尿蛋白检测方面的应用. 用标准光刻腐蚀技术在石英基片上腐蚀泳道, 清洗腐蚀好的基片和盖片后, 在真空条件下实现键合. 此种方法键合制作的电泳微芯片可重复键合使用, 制得的电泳微芯片成功地用于标准蛋白质分离以及临床尿蛋白分析.

关键词: 可逆键合, 电泳微芯片, 尿蛋白

This paper described a low-cost and reliable process for reversible bonding fabrication of electrophoresis microchips on quartz glass substrates in ordinary laboratory without the requirement of clean room facilities, and the microchips have been used in model protein separation and clinical urinary protein detection. Patterns of microchannel were fabricated through standard photolithography and etching on the quartz glass substrate. After bonding surfaces were rigorously rinsed, bonding was realized in vacuum ovens. The chip could be separated and re-bonded for usage. The microchip in these studies was designed to realize on-chip separation of urinary proteins with UV detection. The electrophoretic buffer was 75 mmol/L borate with 0.5 mol/L lactate and pH 10.5. The chips have been used successfully for separation of model proteins and clinical urinary proteins.

Key words: reversible bonding, electrophoresis microchip, urinary protein