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化学学报
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化学学报 ›› 2006, Vol. 64 ›› Issue (24): 2467-2472. 上一篇    下一篇

研究论文

甘草多糖螺旋结构的原子力显微镜研究

孙润广*,1, 张静2   

  1. (1陕西师范大学物理学与信息技术学院生物物理与生物医学工程研究室 西安 710062)
    (2陕西师范大学食品工程系 西安 710062)
  • 投稿日期:2006-06-12 修回日期:2006-12-01 发布日期:2006-12-28
  • 通讯作者: 孙润广

A Study of Helical Structure of Glycyrrhiza Polysaccharides by Atomic Force Microscope

SUN Run-Guang*,1; ZHANG Jing2   

  1. (1 Laboratory of Biophysics and Biomedical Technology, College of Physics and Information Technology, Shaanxi Normal University, Xi'an 710062)
    (2Department of Food Engineering, Shaanxi Normal University, Xi'an 710062)
  • Received:2006-06-12 Revised:2006-12-01 Published:2006-12-28

PDF

1499

被引次数

201 | 29

用原子力显微镜(AFM)对甘草多糖的微观结构进行观察, 实验结果表明, 甘草多糖主要由葡萄糖、阿拉伯糖和半乳糖组成. 甘草多糖分子的稀溶液铺展在Ni2＋处理的云母片上, 经干燥, 乙醇固定后, 获得稳定、重复的图像. 甘草多糖分子具有高度分枝的结构, 并且糖链间形成环状、柱状或近似于螺旋状的结构. 甘草多糖链呈多股紧密的螺旋结构, 这种现象可能与该多糖中分子间的Van der Waals相互作用以及糖链间氢键缔合有关.

关键词: 原子力显微镜, 甘草多糖, 螺旋结构

A method has been developed to give topography of glycyrrhiza polysaccharides by atomic force microscopy (AFM). The results showed that glycyrrhiza polysaccharides mainly consist of glucose, arabi-nose, galactose, but the conclusions about the main chains are different. Aqueous solutions of the polysac-charides are deposited as drops onto Ni2＋ mica surfaces, air dried, and ethanol fixed, and then stable and reproducible images were captured. Glycyrrhiza polysaccharides have a multi-branched structure and a variety of different linkages between adjacent monosaccharides, which compose the small rings and the helical structure. From the images, glycyrrhiza polysaccharides form ordered multiple helical structure. The phenomenon may be related to van der Waals interaction and hydrogen bond of polysaccharide chains.

Key words: atomic force microscopy, glycyrrhiza polysaccharide, helical structure