关键词: 反应动力学, 缩聚, 环糊精, 苯醌, 高聚物, 酶电极, 戊二醛, 氢醌

The effect of soluble α-cyclodextrin prepolymer (CD) on the redox behavior of benzoquinone/hydroquinone (BQ/HQ) system at a platinum electrode was studied and according to the voltammetric curves the inclusion complexation of the poly(α-cyclodextin) host with benzoquinone guest was discussed. The insoluble polymer (PCD) membrane, prepared from polycondensation of CD with glutaric dialdehyde, was used for the immobilization of glucose oxidase (GOD) so that a new second-genernation glucose electrode was obtained. Owing to the inclusion complexation, the benzoquinone as the electron acceptor of reduced GOD in the enzyme-containing polymer membrane has higher concentration, thereby faciliating the electron transfer of the immobilized enzyme. The kinetics of BQ reaction in the immobilized enzyme PCD/GOD matrix was determined.

Key words: REACTION KINETICS, POLYCONDENSATION, CYCLODEXTRIN, BENZOQUINONE, HIGHPOLYMER, ENZYME ELECTRODES, PENTANEDIAL, HYDROQUINONE