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化学学报
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化学学报 ›› 2008, Vol. 66 ›› Issue (12): 1455-1459. 上一篇    下一篇

研究论文

基于金属离子螯合的压电免疫传感器新型固定化方法

陈 媛 吴朝阳* 沈国励 俞汝勤   

  1. (湖南大学化学化工学院 化学生物传感与计量学国家重点实验室 长沙 410082)
  • 投稿日期:2007-11-19 修回日期:2008-03-21 发布日期:2008-06-28
  • 通讯作者: 吴朝阳

A New Metal Ion Chelation Based Immobilization Approach for Piezoelectric Immunosensors

CHEN, Yuan WU, Zhao-Yang* SHEN, Guo-Li YU, Ru-Qin   

  1. (State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering,
    Hunan University, Changsha 410082)
  • Received:2007-11-19 Revised:2008-03-21 Published:2008-06-28
  • Contact: WU, Zhao-Yang

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被引次数

1

提出了一种基于金属离子螯合作用的压电免疫传感器新型固定化方法. 先在压电石英晶振表面沉积正丁胺等离子体聚合膜(BA-PPF), 再在BA-PPF表面修饰可与金属离子螯合的氨三乙酸基团, 用金属铜离子活化后, 修饰了二乙三胺五乙酸基团的IgG抗体蛋白质分子即可螯合固定于BA-PPF上. 将固定了抗体的压电石英传感器用于正常人免疫球蛋白IgG (NHIgG)的测定, 其频率响应与NHIgG浓度在0.36～63.8 μg/mL范围内存在良好的线性关系. 这种新型压电免疫传感器固定化方法简单快速, 具有良好的通用性.

关键词: 等离子体聚合膜, 金属离子螯合, 固定化方法

A new antibody immobilization approach has been proposed for the piezoelectric immunosensors through metal ion chelation. The piezoelectric quartz crystal surfaces were first coated with the plasma-polymerized films of butyl amine (BA-PPF) by using a radio frequency plasma deposition technique. The BA-PPFs were modified with amine triacetic acid groups, and the diethylenetriaminepentaacetic acid-modified IgG antibody (DTPA-IgG Ab) was then immobilized through mediating chelation of metal ions. The piezoelectric immunosensor could detect the normal human IgG in the range of 0.36～63.8 mg/mL. This simple and effective immobilization approach is of certain potential to be a common immobilization for piezoelectric immunosensor construction.

Key words: plasma-polymerized film, metal ion chelation, immobilization approach