关键词: 晶体结构, 诱导剂, 抗癌活性, 元素分析, 质谱法, 核磁共振谱法, 四乙酰己二胺, 二烟酰己二胺, 哌嗪P, 己烷P

Four effective differentiation inducers, the derivatives of HMBA, N, N, N', N'-tetracetylhexamethylenediamine, N, N'-hexamethylenebis (3- pyridinecarboxamide), 4,4'-(hexane-1, 6-diyl)bis(piperazine-2,6-dione) and 3,3'-(hexane-1,6-diyl) bis (5,5-dimethylhydantoin), have been synthesized and identified by MS, NMR, IR and elemental analysis. The synthetic methods and routes have been optimized. The anticancer activity of 4,4'-(hexane-1,6-diyl)bis(piperazine-2,6-dione) against human erythroleukemia K562 cells was tested and its potency is ten times greater than ICRF-154[4,4'-(ethane-1,2-diyl)bis (piperazine- 2, 6-dione)].The structure of compound Ⅳ was determined by single crystal X-ray diffraction and compared with compoundsⅠ,Ⅱ, Ⅲ. In the structures of these compounds, the hexamethylene chain is fully extended, which can increase the flexibility of the molecule. There are hydrogen bonds in compounds Ⅱ,Ⅲ,Ⅳ. It is found that the hydrogen bonds and the ring structure of the functional groups can influence the activity of the compounds.

Key words: CRYSTAL STRUCTURE, ANTINEOPLASTIC ACTIVITY, ELEMENTAL ANALYSIS, MASS SPECTROGRAPHY, NMR SPECTROMETRY, PIPERAZINE P, HEXANE P