化学学报 ›› 2005, Vol. 63 ›› Issue (4): 327-331. 上一篇    下一篇

研究论文

重组质粒对大肠杆菌HB101生长影响的微量热研究

刘 义*,1,王戈林2,赵儒铭1,沈 萍2,屈松生1   

  1. (武汉大学 1化学与分子科学学院 2生命科学学院 武汉 430072)
  • 投稿日期:2004-04-19 修回日期:2004-10-20 发布日期:2010-12-10
  • 通讯作者: 刘 义

Microcalorimetric Study on the Growth of Escherichia coli HB101 Effected by Recombined Plasmid

LIU Yi*,1,WANG Ge-Ling2,ZHAO Ru-Minga   

  1. (1 College of Chemistry and Molecular Sciences, 2 College of Life Sciences, Wuhan University, Wuhan 430072)
  • Received:2004-04-19 Revised:2004-10-20 Published:2010-12-10
  • Contact: LIU Yi

用微量热方法研究了嗜麦芽假单胞菌AT18, 受体菌大肠杆菌HB101, mel基因工程菌——大肠杆菌HB101/pWSY8和携带克隆载体pUC18质粒的大肠杆菌HB101等的生长代谢过程. 实验结果从热化学和热动力学上阐明了细菌的生长速率常数与其所含质粒的大小呈负相关. 探讨了低温处理对含不同质粒大肠杆菌生长的影响, 发现低温处理对工程菌生长影响最大.

关键词: 微量热, 质粒, mel基因, 基因工程菌

The growth metabolism of Pseudomonas maltophilia (P. maltophilia) AT18, Escherichia coli HB101, E. coli HB101/WSY (containing mel gene) and E. coli HB101/pUC18 was studied by means of microcalorimetry. The experimental results show that bacterial growth metabolism is correlated with the sizes of plasmid, and low temperature treatment has obvious effect on the growth of engineering bacteria E. coli HB101/WSY.

Key words: microcalorimetry, recombined plasmid, mel gene, gene engineering bacteria