关键词: 分子印迹技术, 印迹复合膜, Scatchard分析, 渗透, 烟酸

Nicotinic acid molecularly imprinted composite membranes were developed using surface modified polymerization by UV irradiation in the presence of nicotinic acid as template, followed by a deposition of molecularly imprinted polymer layer on the surface of polyvinylidene fluoride microfiltration membranes. Scanning electron micrography (SEM) was used to visualize the surface structure of the imprinted composite membranes. Scatchard analysis shows that equivalent binding sites are formed in the molecularly imprinted composite membranes in the range of investigative concentrations, and the dissociation constant (K_d) of binding sites is 5.55×10^－2 mmol•L^－1. The binding and permeation experiment for substrates indicates that the molecularly imprinted composite membranes exhibit preferable binding performances for nicotinic acid, and the combining quantity is 6.10 μmol•g^－1. Compared with nicotinamide with a similar structure to nicotinic acid, the molecularly imprinted composite membranes show a good selectivity and intense recognition of the template molecule.

Key words: molecularly imprinted technology, imprinted composite membrane, Scatchard analysis, permeation, nicotinic acid