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化学学报
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化学学报 ›› 2010, Vol. 68 ›› Issue (20): 2099-2103. 上一篇    下一篇

研究论文

溶菌酶在羊毛表面的静电自组装固定化及其抗菌性能

王强*，高笑笑，范雪荣，袁久刚   

  1. (江南大学生态纺织教育部重点实验室 无锡 214122)
  • 投稿日期:2010-01-04 修回日期:2010-03-16 发布日期:2010-06-03
  • 通讯作者: 王强 E-mail:qiang_wang@163.com
  • 基金资助:

    国家863计划

Immobilization of Lysozyme on Wool Surface via Layer-by-layer Electrostatic Self-assembly and Its Antibacterial Property

WANG Qiang, GAO Xiao-Xiao, FAN Xue-Rong, YUAN Jiu-Gang   

  1. (Key Laboratory of Eco-Textile of Ministry of Education, Jiangnan University, Wuxi 214122)
  • Received:2010-01-04 Revised:2010-03-16 Published:2010-06-03
  • Contact: Qiang Wang E-mail:qiang_wang@163.com

PDF

1277

被引次数

15

通过层层静电自组装法将溶菌酶和聚苯乙烯磺酸钠(PSS)交替沉积在羊毛纤维表面, 赋予其抗菌性. 应用扫描电镜(SEM)考察组装后纤维表面形态, Zeta电位、染色、溶菌酶活性测试研究纤维表面组装机制并考察改性羊毛抗菌效果. SEM结果表明组装后纤维表面粗糙, 部分凹槽被填埋, 证明溶菌酶/PSS自发组装到纤维表面. 表面电位、染料上染量、溶菌酶活力随纤维表面沉积物质不同, 均呈现明显的“层层交替振荡”现象, 证实了组装后纤维表面电荷的交替变化|织物紫外线透过率随组装层数增加呈近似线性降低, 表明溶菌酶在羊毛表面实现了多层组装, 揭示了羊毛纤维表面抗菌功能涂层的层层静电自组装构筑机制. 同时, 组装层数增多, 溶菌酶活性逐渐增大. 载酶羊毛抗菌率达到91.7%, 具有良好的抗菌性能.

关键词: 溶菌酶, 固定化, 静电自组装, 抗菌, 羊毛

Layer-by-layer electrostatic self-assembly deposition of lysozyme and poly(sodium 4-styrene- sulfonate) (PSS) on wool fibers which endowed them with antibacterial properties were studied. Scanning electron microscopy (SEM) presented the surface morphologies of assembled wool. Zeta potentials, dyeing, activity of lysozymes revealed the mechanism of layer-by-layer electrostatic self-assembly process on fibers and the antibacterial effects were determined. SEM showed rougher surfaces of modified wool fibers with partially-filled grooves, indicating that lysozyme and PSS were assembled on the surfaces of wool fibers. The electrical potential, dye uptake, activity of lysozyme all showed obvious “layer-by-layer alternative oscillation”, which demonstrated the changes of electrical charges on the surfaces of assembled fibers|an approximately linear decrease in UV transmissivity with the increasing assembled layers indicated that lysozymes were multilayeredly assembled on the surface of wool, these revealed the fabrication mechanism of layer-by-layer electrostatic self-assembly antibacterial coatings on fibers surfaces. Meanwhile, the activity of lysozyme was gradually increasing with the increase in the layer numbers of assembled enzyme. The antimicrobial ratio of assembled wool could reach 91.7%, indicating a good antimicrobial property.

Key words: lysozyme, immobilization, electrostatic self-assembly, antibacterial, wool