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化学学报
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化学学报 ›› 2010, Vol. 68 ›› Issue (24): 2564-2568. 上一篇    下一篇

研究论文

荧光光谱法研究法莫替丁-Pd(II)-卤代荧光素染料的相互作用及其分析应用

陈佩丽，刘绍璞，刘忠芳，胡小莉*   

  1. (西南大学 发光与实时分析教育部重点实验室 化学化工学院 重庆 400715)
  • 投稿日期:2010-06-02 修回日期:2010-07-12 发布日期:2010-08-30
  • 通讯作者: 胡小莉 E-mail:xiaolihu@swu.edu.cn;
  • 基金资助:

    生物大分子间相互作用和分子识别的共振瑞利散射和共振非线性散射光谱研究;发光与实时分析重庆市重点实验室项目

Study on the Interaction between Palladium(II)-famotidine and Halogen-fluorescein Dyes by Fluorescence Quenching Method and Their Analytical Applications

CHEN Pei-Li, LIU Shao-Pu, LIU Zhong-Fang, HU Xiao-Li   

  1. (Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715)
  • Received:2010-06-02 Revised:2010-07-12 Published:2010-08-30

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被引次数

2

采用荧光光谱法和吸收光谱法研究法莫替丁(FMTD)与Pd(II)及卤代荧光素染料的相互作用. 在pH 3.2～4.1的NaOAc-HOAc介质中, FMTD与Pd(II)形成螯合阳离子, 它能进一步与二溴荧光素(DBF)、曙红Y (EY)、乙基伊红(EE)和荧光桃红(TCBF)等卤代荧光素(HF)类染料反应形成三元离子缔合物[Pd(FMTD)]•(HF)2, 引起DBF, EY, EE和TCBF吸收光谱变化和荧光猝灭. 离子缔合物的最大吸收峰位于541 (DBF体系)、547 (EY体系)、549 (EE体系)、558 (TCBF体系) nm, 最大荧光发射波长(λem)在513～540 nm范围, 荧光猝灭程度(ΔF)顺序为DBF＞EY＞EE＞TCBF. ΔF在一定的范围内与FMTD的浓度成正比, 检出限为13.6～29.6 ng•mL－1. 据此提出了灵敏度高、选择性好、快速准确测定FMTD的荧光光谱新方法. 可用于服用FMTD后尿药浓度的测定, 为FMTD药代动力学研究提供借鉴. 文中还对三元离子缔合物的组成、结构和荧光猝灭机理进行了讨论.

关键词: 荧光光谱, 法莫替丁, 钯(II), 二溴荧光素, 曙红Y, 乙基伊红, 荧光桃红

The interaction between palladium(II)-famotidine and halogen-fluorescein dyes (HF) was investigated by fluorescence and absorption spectrum. In pH 3.2～4.1 NaOAc-HOAc buffer medium, famotidine (FMTD) reacted with palladium(II) to form chelate cation, which further reacted with dibromofluorescein (DBF), eosine (EY), eosin (EE) and tetrabromotetrachloroflurescein (TCBF) to form ternary ion-association complexes [Pd(FMTD)]•(HF)2. As a result, the absorption bands of DBF, EY, EE, TCBF were shifted and the fluorescences of them were quenched. The maximum absorption wavelengths of [Pd(FMTD)]•(HF)2 were located at 541 (DBF system), 547 (EY system), 549 (EE system), 558 (TCBF system) nm and the maximum fluorescence emission wavelengths of them were located at 513～540 nm, the order of quenching values (ΔF) was DBF＞EY＞EE＞TCBF. The ΔF was proportional to the FMTD concentration in a certain range and the detection limit was 13.6～29.6 ng•mL－1. Based on it, a high sensitive, good selective, simple, rapid and new fluorescence quenching method had been proposed to determine FMTD. It can be applied to determine FMTD in human urine after taking the medicine, which provide reference for pharmacokinetics of the FMTD. The composition and structure of [Pd(FMTD)]•(HF)2 and fluorescence quenching mechanism were fully discussed.

Key words: fluorescence spectrum, famotidine, palladium(II), halogen-fluorescein

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